【摘要】 目的研究缺氧缺血性脑病（HIE）新生儿T辅助细胞（Th）亚群功能失衡的特点,探讨Th₁和Th₂类细胞因子与HIE严重程度之间的关系。方法酶联免疫吸附试验方法,对35例HIE患儿和15例健康对照者外周血单个核细胞（PBMO）经植物血凝素（PHA）刺激后,对培养上清液中Th₁和Th₂类细胞因子含量进行测定。结果经（PHA）刺激后,HIE组Th₁产生IFN-γ、IL-2水平（中位数）明显低于正常对照组（IFN-γ：106 pg／ml比427 pg／ml,P＜0.01。IL-2：527 pg／ml比1218 pg／ml,P＜0.01）,而Th₂产生IL-4、IL-6水平（中位数）则明显高于正常对照组（IL-4：248 pg/ml vs．112 pg／ml,P＜0.05。IL-6：643 pg／ml vs．86 pg／ml,P＜0.01）。相关分析发现：IL-6水平与HIE严重程度呈显著正相关,r=0.474,P＜0.01；IL-2、IFN-γ水平与HIE严重程度呈负相关,r分别为-0.352和-0.357,P值均＜0.05；IL-4水平与HIE严重程度无相关性,r=0.277,P＞0.05。结论HIE新生儿Th₁类细胞因子水平明显降低,Th₂类细胞因子水平明显增高,导致T辅助细胞亚群功能失衡,此在HIE的发病机制中起重要作用。Th类细胞因子水平与HIE严重程度相关。更多还原

【Abstract】 Objective To study the dysfunctional feature of T helper cell subsets in neonatal hypoxic-ischemic encephalopathy（HIE）, and explore the relationship between the cytokines of Th₁, Th₂ and the degree of HIE. Methods The cytokine production in the cultured supernatants of peripheral blood mononuclear cells （PBMC） stimulated with PHA, from 35 neonates with HIE and 15 age-matched healthy controls, was determined using "sandwich" ELISA. Results The Th₁ cytokine levels （median） of IFN-γ and IL-2 of HIE group were statistically lower than those in controls, IFN-γ: 106 pg/ml vs. 427 pg/ml, P＜0.01; IL-2: 527 pg/ml vs. 1218 pg/ml,P＜0.01. In contrast, the Th₂ cytokine levels （median） of IL-4 and IL-6 of HIE group were obviously higher compared with the controls, IL-4: 248 pg/ml vs 112 pg/ml,P＜0.05; IL-6: 643 pg/ml vs. 86pg/ml , P＜0.01. Levels of IL-6 in patients with HIE were markedly positive correlated with the degree of HIE, r=0.474, P＜0.01. The levels of IL-2 in HIE group were negative correlated with the degree of HIE, r=-0.352, P＜0.05. IFN-γ in HIE group was negative correlated with the degree of HIE, r=-0.357, P＜0.05. IL-4 in patients with HIE had no correlation with the degree of HIE, r=0.277, P＞0.05. Conclusions Obviously low levels of Th₁ cytokine and high levels of Th₂ cytokine in HIE cause the dysfunctional of T helper cell subsets, Which might play an important role in the pathogenesis of HIE. Levels of Th cytokine were markedly correlated with the degree of HIE.更多还原