【摘要】 鸡毒支原体(MG)是造成鸡慢性呼吸道疾病的主要病原菌。通常采用血清学方法诊断该病,由于存在非特异性交叉反应,这给临床诊断带来困难。为解决MG快速诊断问题,该研究根据已发表的鸡毒支原体种特异性序列FMG-2合成一对引物(MG1,MG2),用PCR方法检测MG。结果表明,该PCR方法对MG能特异性扩增732bp目的片断,而对照菌(大肠杆菌、金黄色葡萄球菌等)却不能扩增出目的片段。PCR产物经测序,其序列与Genbank序列同源性为98.86%。用PCR方法检测60份感染样本,其阳性检出率为80%,而用传统的分离培养方法检出率仅为50%。更多还原

【Abstract】 Mycoplasma gallisepticum(MG) is the etiologic agent of avian chronic respiratory disease. Traditional serologic test for the diagnosis of the disease has encountered with cross-reaction. Therefore, it is difficult to diagnose MG infection with serologic methods. In order to solve this problem ,a PCR method based the reported DNA sequence of FMG-2 to detect MG was developed. A 732-bp expected fragment was amplified from MG DNA, but it was not amplified from other bacteria(including E.coli,S.aureus,MS,et al ). Products of PCR from a reference strain and an isolate were sequenced .In comparison with the sequences in GenBank, the homology was 98.86%.Among the 60 clinical samples, the positive ratio was 80% for PCR, but the positive ratio was only 50% for isolation method.更多还原