【摘要】 本文以鸭茅基因组DNA为模板,利用小麦SSR引物对鸭茅SSR反应体系中的一些重要参数进行摸索和优化实验,建立了一套鸭茅SSR分子标记的最优化反应体系,在15μl体系中,最终浓度或量:Mg2+为2.5 mm/L、dNTP为400μmol/L、Taq酶为1.0U、引物浓度为2.0~2.5μpmol/L、模板NDA量为60~80 ng。同时成功的筛选出20对能较好的应用于鸭茅种质资源遗传多样性研究的SSR引物,可用于鸭茅SSR标记的进一步研究。更多还原

【Abstract】 Based on the genomic DNA extracted from Dactylis glomerata and SSR primers of wheat,some essential factors that might affect the result of SSR assay were tested and compared.An optimal reaction system suitable for the assay and usage of SSR in Dactylis glomerata was established.There are 2.5 mm/L Mg²⁺,400μmol/L DNTP,1.0 U Taq DNA polymerase,2.0-2.5μpmol/ L primer,60-80 ng template DNA in a volume of 15μl amplification reactions system.Twenty SSR primers of wheat were selected,which could be used in Dactylis glomerata.更多还原