【摘要】 利用十二烷基磺酸钠-聚丙烯酰胺凝胶电泳技术(SDS-PAGE)对106份人工合成小麦(2n=6x=42,AABBDD)的高分子量谷蛋白亚基(HMW-GS)组成进行了分析。共检测到27种亚基类型,其中Glu-D1位点上的变异类型最为丰富,发现了包含一种未知亚基及新的亚基组合在内的18种不同类型。分析发现:Glu-A1,Glu-B1和Glu-D1三个位点均编码与小麦面包烘烤品质密切相关的优质亚基类型,如2*,7+8,14+15,5+10,5+12亚基。因此,本批材料可能对小麦的品质改良具有重要的应用价值。更多还原

【Abstract】 The compositions of high molecular weight glutenin subunits(HMW-GS)in 106 synthetic wheats were analyzed by using sodium dodecyl sulphate polyacrylamide gel electrophoresis(SDS-PAGE).There were 27 different HMW-GS types,in which eighteen allelic variations including an unknown novel subunit and some new HMWGS compositions were detected at Glu-D1 locus.Meanwhile,several subunits important for quality breeding were identified at three loci(Glu-A1,Glu-B1 and Glu-D1),such as 2*,7+8,14+15,5+10,5+12 and so on.Therefore these synthetic wheats will be very useful for improving the wheat quality.更多还原