【摘要】 为了探讨微量元素锌和硒互作对肉鸡肠道黏膜屏障结构的影响,将24只1日龄AA肉鸡随机分3组,分别饲喂添加有高锌高硒(锌1 000 mg/kg、硒5 mg/kg)、低锌低硒(锌34 mg/kg、硒0.08 mg/kg)或常锌常硒(锌50mg/kg、硒0.15 mg/kg)的日粮45 d后,用免疫组化方法检测肠道内IgA+浆细胞及阳性反应物的数量及分布变化。结果表明:IgA+浆细胞及阳性物质散在分布于小肠绒毛顶端,固有层、肠腺周围以及盲肠黏膜固有层和盲肠扁桃体的淋巴小结周围。在常锌常硒组,IgA+浆细胞的数量及IgA阳性反应物比较多,而低锌低硒组和高锌高硒组的IgA+浆细胞数量及IgA阳性反应物数量减少(P<0.01),尤其高锌高硒组减少更为明显。结论:低硒低锌或高硒高锌可抑制肉鸡肠道IgA形成,影响肠黏膜的免疫功能。日粮中按50 mg/kg锌、0.15 mg/kg硒的比例添加,对于维持正常肠道黏膜免疫功能是合适的。更多还原

【Abstract】 To explore the effect of trace elements zinc,selenium and their interaction on the gut barrier function in the broiler,twenty-four one-day-old AA broilers were divided into three groups,and were fed with the different diets including high zinc-selenium(Zn 1 000 mg/kg,Se 5 mg/kg),low zinc-selenium(Zn 34 mg/kg,Se 0.08 mg/kg),and normal zinc-selenium(Zn 50 mg/kg,Se 0.15 mg/kg),respectively.After forty-five days,the broilers were killed and the distribution and quantity of IgA~+-cell and IgA positive reaction substance in the gut were observed by immunohistochemistry.The results showed that the IgA~+-cell and IgA positive reaction substance were located in the tip of villus,lamina propria,surround intestinal gland,and lymphatic nodule of cecum.In two groups of high zinc-selenium and low zinc-selenium,the quantity of IgA~+-cell and IgA positive reaction substances were decreased(P<0.01),especially in the high zinc-selenium group.The distribution and the number of the IgA~+-cell and IgA positive reaction substances were normal in the normal zinc-selenium group.This study indicated that the low zinc-selenium or high zinc-selenium would repress production of IgA,and the supplementary level of selenium at 0.15 mg/kg and zinc at 50 mg/kg in diet should be an appropriate ratio which would keep the normal gut barrier function in the broiler.更多还原