【摘要】 克隆了鸡Camk2d基因的部分片段并发现Camk2d基因第15外显子51bp处有一C→T碱基转换的沉默突变,该突变位点具有VspI酶切多态,通过PCR-RFLP技术对我国乌骨鸡、鹿苑鸡、固始鸡、白耳鸡、仙居鸡、斗鸡和大骨鸡等7个不同地方品种鸡进行了基因型检测。结果表明:在7个品种中存在3种基因型(CC、CT、TT),χ2检验显示在各品种间基因型存在不同差异。同时在藏鸡,隐性白羽鸡,杂交鸡(藏♂×隐♀)和75%血液藏鸡[藏♂×(藏♂×隐♀)♀]群体中进行了基因-性状关联分析,结果表明,该SNP位点与腹脂存在极显著相关(P<0.01),与肌肉pH值显著相关(P<0.05)。更多还原

【Abstract】 In this study,we cloned part DNA sequence of chicken Camk2d gene and found a C→T silent mutation at 51th bp in exon 15.Genotypes were detected by VspI-PCR-RFLP in 7 different native chicken lines in this test.The result of Chi square showed there had 3 genotypes(CC,CT,TT) in these 7 lines,and we could find the different distribution of three genotypes in different lines.According to the general linear model,we analyzed the correlation between gene and character among the three genotypes of Camk2d gene in 4 breeds(combinations).The result of variance analysis showed that there had significant correlation between this SNP site and abdominal fat(P<0.01),and correlation between this SNP site and pH value(P<0.05).更多还原