【摘要】 研究了切割取样后小鼠胚胎和牛胚胎冷冻-解冻的技术方法。结果表明:(1)分别以10%甘油、10%乙二醇作为冷冻液以及在以上两种冷冻液中分别添加10%葡聚糖+0.1 mol/L蔗糖作为冷冻液常规方法冷冻切割后的小鼠胚胎,冷冻-解冻后体外培养72 h总发育率分别为56.9%(259/455)、81.8%(604/738)、84.8%(540/637)和59.5%(308/518)。(2)取样后胚胎体外短暂培养(0~4 h)并不能提高切割取样后小鼠胚胎冷冻-解冻体外培养发育率。(3)切割取样小鼠胚胎在25%甘油+0.25 mol/L蔗糖+20%的血清为冷冻液,在-100~-110℃左右熏蒸2 min快速冷冻后体外培养发育率为73.3%(33/45),切割取样牛胚胎快速冷冻-解冻移植妊娠率为57.1%(4/7)。更多还原

【Abstract】 Cryopreservation technique of biopsied mouse and bovine blastocysys were also studied.As a result,(1) The biopsied mouse embryos were freezed with 10% glycerol and 10% glycol respectively and,as comparison,the biopsied mouse embryos were freezed with both of the freezing solution added 10% polysaccharid and 10%sucrose,the development rate of frozen-thawed mouse embryos were 56.9%(259/455),81.8%(317/390),84.8%(540/738),59.5%(308/518) respectively.(2)The development rate of frozen-thawed mouse embryos could not be improved when the biopsied embryos were cultured in vitro shortly(about 0-4 hours) after sampling.(3)Being suffocated 2 minutes at-100 ℃——110 ℃ after sampling and fast cryopreservation with frozen solution which include 25% glycerol and 0.25 mol/L sucrose and 20% blood serum,the development rate of muse embryos was 73.3%,the pregnancy rate of fast frozen-thawed bovine embryos after sampling was 57.1%(4/7).更多还原