【摘要】 从临床病料中分离鉴定出1株无血凝性的致病性兔出血症病毒(RHDV),命名为Yaan-1。HA试验测得该毒株原代及经兔体传3代后的肝毒在25℃、37℃和4℃时均无血凝性;但在琼脂扩散试验和对流免疫电泳中均可见分离株与常规RHDV高免血清形成清晰的沉淀线;电镜观察可见30 nm左右的病毒粒子。采用RT-PCR方法将Yaan-1株的衣壳蛋白VP60基因进行了克隆测序,序列分析表明VP60基因序列全长1 740 bp,编码579个氨基酸,测序结果提交GenBank(注册号为DQ069280),并与世界各国的常规RHDV分离株进行比较,结果表明核苷酸同源性为90.0%~98.0%,氨基酸同源性为94.1%~99.0%,氨基酸变异多发生在衣壳蛋白C、E区;同时对Yaan-1株的分子量、等电点、疏水性、跨膜区等分子结构特征进行了分析。本研究首次报道了无血凝性RHDV中国分离株,丰富了地方毒株资源,为明确我国地方株的分子流行病学,以及对VP60蛋白分子特征研究提供了新材料。更多还原

【Abstract】 A variant strain of rabbit hemorrhagic disease virus which was named "Yaan-1" was(isolated) and identified in China.The virus lacked HA activity at 25,37 and 4 ℃,but gave positive results in agar diffusion reaction(ADR) and counter immunoelectrophoresis(CIE).The capsid protein(VP60)of Yaan-1 strain was cloned by RT-PCR and sequenced.VP60 gene of Yaan-1 was 1 740 nt in size and encoding 579 aa.Alignment with other 14 sequences of RHDV isolates in the world showed that the homology were 90.0%-98.0% for nucleotide sequence,and 94.1%-99.0%for amino acid sequence respectively.The results indicated that the sequence of VP60 gene of different RHDV isolates,including non-haemagglutinating Yaan-1 strain,were highly homologous.The hydrophilicity plot,flexible regions,antigenic index of VP60 protein of Yaan-1 strain,etc.were also analyzed.更多还原