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人参皂甙Rb1和黄芪多糖对微血管内皮细胞分泌NO、IL-6和TNF-α的影响

Effect of Ginsenoside Rb1 and Astragalus Polysaccharide on the Excretion of NO,IL-6 and TNF-α in Micro-vascular Endothelial Cells

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【Author】 XIE Hui-mei~(1,2),HU Ge~2,SUO Zhan-wei~2,LIU Yi-tong~1,YIN Long~2,YU Tong-quan~2,LU Ping~2,MU Xiang~(2*),HUANG Hui-ling~(1*)(1.College of Animal Science and Technology,Agricultural Univertsity of Hebei, Baoding 071001,China;2.Department of Animal Science and Technology, Beijing Agricultural College,Beijing 102206,China)

【机构】河北农业大学动物科技学院；北京农学院动物科技系；河北农业大学动物科技学院保定071001；北京102206；保定071001；

【摘要】取中药提取物人参皂甙Rb1和黄芪多糖作用于体外培养大鼠肠黏膜微血管内皮细胞,用硝酸还原酶法测定一氧化氮(NO)、ELISA法测定白介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)水平的变化,为分析其免疫作用机制提供试验依据。微血管内皮细胞在人参皂甙Rb1和黄芪多糖的作用下,细胞分泌NOI、L-6和TNF-α均增多,并且随着浓度的增加内皮细胞分泌量也增加。更多还原

【Abstract】 Two Chinese herbal medicine extraction,ginsenoside Rb1 and astragalus polysaccharide,were supplemented into in vitro cluture system of rat micro-vascular endothelial cells.NO was detected by reduction of nitrate method and IL-6,TNF-α were detected by ELISA,the changes of those relevant factors provided experimental evidence for analyzing effect of ginsenoside Rb1 and astragalus polysaccharide.The level of NO,IL-6 and TNF-α in micro-vascular endothelial cells were enhanced significantly by ginsenoside Rb1 and astragalus polysaccharide,and showed concentration-dependent trend.更多还原

【关键词】黄芪多糖；人参皂甙Rb1；微血管内皮细胞； NO； IL-6； TNF-α；
【Key words】 astragalus polysaccharide； ginsenoside Rb1； micro-vascular endothelial cells； nitric oxide； IL-6； TNF-α；

【基金】农业应用新技术北京市重点实验室(KF2003-1)

【文献出处】畜牧兽医学报 ,Acta Veterinaria et Zootechnica Sinica , 编辑部邮箱 ,2006年09期

【分类号】R285
【被引频次】46
【下载频次】736

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