【摘要】 以骨形态发生蛋白受体IA(bone morphogenetic protein receptor IA,BMPR-IA)基因为候选基因,采用PCR-SSCP技术检测该基因在高繁殖力绵羊品种(小尾寒羊、湖羊)以及低繁殖力绵羊品种(多赛特羊、特克塞尔羊、德国肉用美利奴羊)中的单核苷酸多态性,同时研究该基因对小尾寒羊高繁殖力的影响。结果表明:在小尾寒羊中检测到AA、AB两种基因型,在湖羊中只检测到一种基因型BB,而在低繁殖力的3个绵羊品种中只检测到一种基因型AA。统计结果表明:小尾寒羊A等位基因频率为0.964,B等位基因频率为0.036。测序结果表明:BB型与AA型相比有6处核苷酸发生了突变。独立性检验表明:小尾寒羊与低繁殖力绵羊品种间基因型分布差异不显著(P>0.05),而湖羊与小尾寒羊、低繁殖力绵羊品种间基因型分布差异极显著(P<0.001)。AB基因型小尾寒羊平均产羔数比AA基因型多0.15只,但差异不显著(P>0.05)。研究表明:BMPR-IA基因不是小尾寒羊和湖羊高繁殖力的主效基因。更多还原

【Abstract】 Bone morphogenetic protein receptor IA(BMPR-IA) gene was studied as a candidate gene for the prolificacy of sheep.Single nucleotide polymorphism of BMPR-IA gene was detected in both high fecundity breeds(Small Tailed Han sheep and Hu sheep) and low fecundity breeds(Dorset sheep,Texel sheep and German Mutton Merino sheep) by PCR-SSCP.Results indicated that both AA and AB genotypes were detected in Small Tailed Han sheep.Only BB genotype was detected in Hu sheep.Only AA genotype was detected in Dorset sheep,Texel sheep and German Mutton Merino sheep.In Small Tailed Han sheep,frequency of A allele was 0.964,frequency of B allele was 0.036.Sequencing revealed six single nucleotide mutations in the BB genotype in comparison to the AA genotype.The BMPR-IA genotype distributions were high significantly different(P<0.001) between Hu sheep and Small Tailed Han sheep.The BMPR-IA genotype distributions were high significantly different(P<0.001) between Hu sheep and low fecundity breeds.The BMPR-IA genotype distributions were not different(P>0.05) between Small Tailed Han sheep and low fecundity breeds. The ewes with genotype AB had 0.15(P>0.05) lambs more than those with genotype AA in Small Tailed Han sheep.These results preliminarily showed that BMPR-IA gene was not a major gene for high fecundity of Small Tailed Han sheep and Hu sheep.更多还原