【摘要】 利用复制缺陷型重组腺病毒作为基因表达载体,将猪生长激素(pGH)基因直接转导到猪体内细胞,研究猪生长激素基因在猪体内的促生长作用。用同源重组的方法,构建含pGH基因的重组腺病毒(Ad-pGH)。将其感染293细胞和PK-15细胞,均可检测到pGH。用1 mL此重组腺病毒(1010TCID50)一次性肌肉注射60日龄的杂交长白猪。结果表明,试验组的猪平均增重比对照组的猪增加37%(注射后第4周)和19%(注射后第6周);饲料报酬提高28%(注射后第4周)和18%(注射后第6周)。试验结果证明,由重组腺病毒表达的pGH具有生物学活性,在猪体内起到明显的促进生长的作用。重组腺病毒介导的pGH基因在猪体内的表达可维持约4周。更多还原

【Abstract】 A recombinant replication-defective human adenovirus serotype 5 containing the porcine growth hormone gene（Ad-pGH） was constructed with a homologous recombination method.A high level expression of pGH was detected in HEK293 cells and PK-15 cells infected with Ad-pGH.Furthermore,Ad-pGH were injected intramuscularly at a dose of 1 mL(10¹⁰TCID₅₀) each to 60 day-old crossbred pigs.Compared with the control animals at 4 weeks and 6 weeks after the injection,the pigs increased in weight gain by 37% and 19% respectively.The feed/gain ratio improved in Ad-pGH treated pigs.These results suggested that Ad-pGH is able to promote growth in pigs significantly.The in vivo expression of pGH can be maintained about four weeks.更多还原