【摘要】 观察了高盐诱发肉鸡腹水综合征(AS)发生发展过程中肺小动脉中膜平滑肌细胞(SMC)表型转化的规律,旨在探讨肺小动脉中膜SMC表型转化在AS发生过程中的可能作用。100只1日龄商品代AA雄性肉仔鸡常规育雏,8日龄起随机分为对照组和高盐组(饮水中添加0.30%氯化钠)。分别在15、22、29、36、43、50日龄时每组随机取5只鸡的肺组织并制备石蜡切片,以免疫组织化学方法结合图像分析法测定SMC表型标志蛋白-αSM-actin的表达。结果表明:(1)对照组肉鸡的生长发育过程中存在肺小动脉中膜SMC表型的动态转化,22日龄时发生了由收缩表型向增殖表型的转化,随后逐步向收缩表型回转,且各级肺小动脉SMC转化时间不一致。(2)高盐组SMC表型转化时间(15日龄)较对照组提前了1周,高盐促进了SMC由收缩表型向增殖表型转化。且直径20~50μm的肺小动脉SMC表型转化受高盐影响最大,推测其在高盐诱发肉鸡AS的过程中可能起更重要的作用。更多还原

【Abstract】 In this study,the rule of phenotypic change of smooth muscle cell(SMC) in the media of pulmonary arterioles was investigated during the development of ascites syndrome(AS) induced by excess salt in broiler chickens.One hundred male Arbor Acres broilers at day 8 were randomly divided into control group(C) and treated group(T).Chickens in the treated group were supplied with drinking water added 0.30% NaCl,and other factors were the same as that in control group.Lung samples of 5 randomly selected birds per group were collected to make routine paraffin section at 15,22,29,36,43 and 50 days respectively.Expression of α-SM-actin(phenotype marker protein of SMC) was determined by immunohistochemistry staining and image(analysis) program.The results suggested that there was dynamic phenotypic change of SMC in the media of pulmonary arterioles of broiler chickens in the control group.At day 22 SMC changed from contractile phenotype to proliferative phenotype,and did again from proliferative phenotype to contractile phenotype with in different outer diameter pulmonary arterioles at different time.Excess salt accelerated change of SMC from contractile phenotype to proliferative phenotype,and phenotypic change of SMC in pulmonary arterioles with 20-50 μm diameter was affected mostly and may play an important role in the development of AS.更多还原