【摘要】 采用超薄切片和透射电子显微镜技术对鸭病毒性肠炎病毒CH强毒株(DEV-CHv)在鸭胚成纤维细胞(DEF)中的形态发生学进行了研究。结果表明,DEV-CHv吸附到DEF细胞上以后通过囊膜和细胞膜间发生融合的方式侵入宿主细胞;病毒在细胞核内完成病毒核酸的生成、核酸与衣壳的装配;病毒核衣壳通过核膜-内质网膜系统从细胞核转运到内质网池中并在内质网池中得到皮层结构;获得了皮层的DEV-CHv核衣壳通过出芽方式释放到细胞空泡内得到囊膜结构后成为成熟病毒;细胞空泡内的成熟DEV-CHv可通过细胞的胞吐作用被释放到细胞外,或在空泡破裂时被释放到细胞外。更多还原

【Abstract】 Morphogenesis of duck enteritis virus CH virulent isolate(DEV-CHv) was investigated by ultrathin sectioning and transmission electron microscopy of infected duck embryo fibroblasts(DEF).Attached DEV-CHv probably penetrated the DEF cell membrane by direct fusion between the viral envelop and the plasma membrane.Progeny nucleocapsids assembled in the nucleus and exited from this compartment into the cytoplasm by budding through the inner nuclear leaflet into the interconnected perinuclear cisterna and cisternae of endoplasmic reticulum.DEV tegumentation occurred in cisternae of endoplasmic reticulum and the tegumented progeny nucleocapsids obtained their final envelop by budding into cytoplasmic vesicles.Intravesicular mature DEV-CHv particles were discharged from the cell through exocytosis of the cytoplasmic vesicles by DEF,or through rupture of the virus-containing vesicles.更多还原