【摘要】 磷酸丝氨酸转氨酶(Phosphoserine aminotransferase,SerB)是L-丝氨酸生物合成中的关键酶,应用PCR技术从大肠杆菌JM109(E.ColiJM109)中扩增出磷酸丝氨酸转氨酶基因,将其与表达载体pEC 7连接。通过电转化方法,将重组质粒转入黄色短杆菌(Brevibacterium flavumC-11,BfC-11)中,经酶活检测和摇瓶发酵培养,含有重组表达质粒的BfC-11B的磷酸丝氨酸转氨酶的活力和L-丝氨酸产率均比原宿主菌BfC-11有所提高,为构建L-丝氨酸高产基因工程菌的研究奠定了基础。更多还原

【Abstract】 Phosphoserine aminotransferase(SerB) is a key enzyme in biosynthesis of L-serine.The SerB gene is obtained from E.coli JM109 by PCR. This gene was inserted into pEC7 and the recombinant plasmids with SerB gene were transformed into Brevibacterium flavum C-11(BfC-11)by the method of electrotransformation and by detecting enzyme activity and the fermentation culture of BfC-11 and BfC-11B.The results showed that the activity of SerB and the yield of L-serine in BfC11B are higher than those in BfC-11.It lays foundations for the research of construction of high L-serine produced gene-engineering strain.更多还原