【摘要】 以油葵自交种7B7的无菌苗子叶为诱导不定芽材料,MS培养基为基本培养基,研究油葵子叶组织培养有关影响因子。比较了不同培养基成分、不同碳源、不同附加物对不定芽分化的影响,确定出油葵自交种子叶离体诱导再生芽的最佳培养基:改良MS(MS大量、微量、铁盐、B5有机)+0.5%KNO3+0.05%CH+0.01%肌醇+3%葡萄糖+4 mg/L 6-BAP+6~9 mg/L AgNO3+2.6 g/L Phytagel,建立了油葵相对高效的再生体系。为油葵基因转化的研究奠定了基础。更多还原

【Abstract】 The related factors of tissue culture were studied by using cotyledon of 7B7,self-hybrided Helianthus annuus L.as inducible material and MS（Murashige and Skoog） as basic medium.The influence of regeneration of different composition of medium,source of carbon,additional substances on adventitious bud diffentiation was compared,the best cultured medium was obtained:MS+0.5%KNO₃+0.05%Casein Hydrolysace+0.01% myo-inositol+30%glucose+4 mg/L 6-BAP+6～9 mg/L AgNO₃+2.6 g/L Phytagel were reformed.The efficient regeneration system was established and the foundation of genetic transformation was laid.更多还原