【摘要】 为了研究碱性-螺旋-环-螺旋(basic-helix-loop-helix,bHLH)转录因子的生物学功能,通过对开源数据库TIGR中水稻基因序列的预测,我们找到了水稻中的部分bHLH转录因子家族成员.用反转录-聚合酶链式反应(RT-PCR)的方法在水稻根、茎、叶、花和种子中,对这些成员的表达模式进行分析,然后与已有的拟南芥的表达信息进行比较.结果显示,部分序列相似度较高的bHLH基因,表达模式也较为相似,说明其在功能上可能存在联系.更多还原

【Abstract】 To study the biological function of the bHLH(basic-helix-loop-helix) transcription factors,according to the prediction result from the rice gene sequences,we obtained some members of the bHLH transcription factor family.RT-PCR was performed to detect the expression pattern of these members in the root,stem,leaf,flower and seed of rice.Then the result was compared to the expression pattern of Arabidopsis.We found that genes with the similar sequences in both Arabidopsis and rice might have similar expression patterns,this indicates that they may have functional similarity.更多还原