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RP-6启动子连接的IPT基因的双元载体构建
The Construction of a Plant Binary Vector pCAMBIA130-pRP-6-IPT-Nos for Expressing IPT Gene in Developing Rice Seed
【摘要】 以含有IPT基因的中间载体pSG516为基础,采用酶切的方法获得IPT-Nos核酸片段,以水稻品种9311为材料,采用PCR的方法克隆出种子中特异表达的醇溶谷蛋白RP-6基因启动子,并将此启动子连接到pCAMBIA1300上,构建pCAMBIA1300-pRP-6载体,将IPT-Nos核酸片段插入到pCAMBIA1300-pRP-6,构建了pCAMBIA1300-pRP-6-IPT-Nos双元表达载体.
【Abstract】 In order to obtain transgenic rice which express exogenous IPT gene in developing seed and to further investigate the function of cytokinin in rice seed development,we constructed the binary vector pCAMBIA1300-pRP-6-IPT-Nos.Firstly IPT-Nos fragment was obtained from pSG516 by cutting PSG516 plasmid with Nco I and Spe I restriction enzymes.Secondly,developing endosperm RP-6 gene specifically expressed in developing endosperm was selected and it′s promoter(pRP-6) was isolated from rice genomic DNA(cultivar 9311) by using PCR amplification.Thirdly pRP6 was inserted into pCAMBIA1300,with pCAMBIA1300-pRP-6 obtained.Finally,plant expression vector pCAMBIA130-pRP-6-IPT-Nos was constructed after inserting IPT-Nos fragment into pCAMBIA1300-pRP-6.
【Key words】 pRP-6； IPT； pCAMBIA1300-pRP-6-IPT-Nos； specific expression；
- 【文献出处】 厦门大学学报(自然科学版) ,Journal of Xiamen University(Natural Science) , 编辑部邮箱 ,2006年03期
- 【分类号】Q943.2;S511
- 【被引频次】2
- 【下载频次】221