【摘要】 目的:宫颈癌的化疗是以铂类药物为基础的化疗,对顺铂和卡铂在宫颈癌化疗中的作用进行体外药敏实验,对其细胞毒性进行比较,为临床选择用药提供实验依据。方法:1.ATP标准曲线的测定;2.采用三磷酸腺苷生物发光法,对宫颈癌细胞株Hela、Caski、Siha进行药敏实验,药物分别为顺铂和卡铂,浓度分别为0.5×、1×和2×血浆峰值浓度。结果:1.ATP标准品在浓度为10-9~10-5mol/ml时,与发光计数值显示极好的线性关系,直线方程为Y=0.892X+10.257,相关系数r=0.998(P<0.001)。2.在0.5×、1×和2×血浆峰值浓度下,Hela细胞株顺铂作用抑制率分别为53.5%、69.2%、100%,卡铂作用抑制率分别为56.1%、64.2%、98%;Siha细胞株顺铂作用抑制率分别为78.3%、84%、95%,卡铂作用抑制率分别为69.5%、77.8%、98%;Caski细胞株顺铂作用抑制率分别为36%、45%、52.6%,卡铂作用抑制率分别为40%、41.7%、42.3%,经统计学处理差异无统计学意义(P>0.05)。结论:顺铂和卡铂对宫颈癌细胞株的细胞毒性作用无差异统计学意义,由于卡铂毒副作用小,在宫颈癌化疗中优于顺铂。更多还原

【Abstract】 Objective:To evaluate the effect of cisplatin and carboplatin using chemosensitivity testing,and compare their cell toxicity and provide experimental evidence for clinical application.Methods:1.The mensuration of ATP standard curve.2.An ATP-CVA test was used to determine the drug sensitivity of Hela cells、Caski cells、Siha cells.The drug were cisplatin and carboplatin and the concentration were 0.5×PPC,1×PPC and 2×PPC respectively.Results:1.In a range of 10^-9-10^-5 mol/ml,there is a linear relationship between the log ATP and the log luminescence（Y=0.892X+10.257,correlation coefficient r=0.998）（P<0.001）.2.At the concentration of 0.5×PPC、1×PPC and 2×PPC,the restraint rate of cisplatin of Hela cells were 53.5%、69.2% and 100% respectively,the restraint rate of carboplatin of Hela cells were 56.1%、64.2% and 98% respectively;the restraint rate of cisplatin of Siha cells were 78.3%、84% and 95% repectively,the restraint rate of carboplatin of Siha cells were 69.5%、77.8% and 98% respectively;the restraint rate of cisplatin of Caski cells were 36%、45% and 52.6%,respectively,the restraint rate of carboplatin of Caski cells were 40%、41.7% and 42.3%,respectively;the differences were not significant（P>0.05）.Conclusion:The difference of cell toxicity between cisplatin and carboplatin was not significant,and carboplatin is better than cisplatin in the chemotherapy of cervical cancer for its little side effect.更多还原