【摘要】 目的建立肺炎喘嗽口服液中盐酸麻黄碱含量测定方法。方法使用HypersiL C18柱(250mm×4.0mm,5μm),流动相:乙腈-0.02mol.mL-1磷酸二氢钾(含0.2%三乙胺,磷酸调pH2.7)(5∶95),流速1.0mL.m in-1,检测波长210nm。结果盐酸麻黄碱在12.8~76.8μg.mL-1范围内线性关系良好(r=0.999 8),平均回收率为99.85%,RSD=0.27%(n=5)。结论本方法简便,灵敏,准确,重现性好,可用于本制剂的含量测定和质量控制。更多还原

【Abstract】 OBJECTIVE To establish an RP-HPLC method for ephedrine hydrochloride in Feiyan Chuanshu oral solution.METHODS HPLC,column: HypersiL ODS-C18(250mm×4.0mm,5μm),mobile phase: acetonitrine-0.02mol·mL-1potassium dihydrogen phosphate(0.2% triethylamine,adjusted pH to 2.7 with phosphoric acid)(5∶95).The flow rate was at 1.0mL·min-1 and the detective wavelength was at 210nm. RESULTS The calibration curve was linear within a range of 12.8～76.8μg·mL-1 for ephedrine hydrochloride,the average recovery was 99.85% with RSD=0.27%(n=5).CONCLUSION The method is accurate with a good reproducibility and can be used as a quantitative analysis for preparation.更多还原