【摘要】 目的用高效液相色谱法测定金钱草中槲皮苷的含量。方法采用Sh im adzu C18柱(150×4.6mm,5μm),流动相为甲醇-0.2%H3PO4溶液(45∶55),流速1.0mL.m in-1,检测波长350nm,柱温室温,用外标法定量,测定金钱草中槲皮苷的含量。结果槲皮苷的线性范围0.40~2.00μg,r=0.999 6,回收率96.21%,RSD2.91%。结论该方法可用于金钱草中槲斗皮苷的含量测定。更多还原

【Abstract】 OBJECTIVE To determine the content of quercitrin in Lysimachia christinae by HPLC.METHODS The analysis was carried out on Shimadzu C18 column(150mm×4.6mm,5μm).The mobile phase was MeOH-0.2%H3PO4(45∶55).Flow-rate was 1.0mL·min-1.Wave-length was 350nm.Temperature was room temperature. RESULTS The method was simple and had a good linear relationship.The liner range of quercitrin was 0.40-2.00μg and the correlation(r) was 0.9996.The recovery was 96.21% with RSD 2.91%.CONCLUSION The method is easy to determine the content of quercitrin in Lysimachia christinae.更多还原