【摘要】 目的建立快速、有效合成正电子发射体层摄影(PET)示踪剂11C-乙酸的方法并研究其生物分布,满足临床PET的用药需要。方法以11C-CO2为原料,与CH3MgBr反应后再用17%的H3PO4酸解,产物通过加热的方法蒸出,同时通过控制11C-CO2的释放速度和适当延长11C-CO2与CH3MgBr的反应时间,提高反应产率。小鼠给药后不同时间处死,分别取不同器官称重并测放射线计数。结果从加速器生产出11C-CO2到产物11C-乙酸的蒸出完毕,需15min,放化纯度大于98%,化学纯度大于99%,平均产率为34.6%(未经衰变校正)。注射11C-乙酸后,放射线主要集中在肝脏及肾脏。结论改进反应条件后的合成方法具有快速、高效的特点,为PET日常耗材节省了成本,提高了用药的质量。更多还原

【Abstract】 Objective To establish a rapid,effective method for the synthesis of 11C-acetate and study the biodistribution of 11C-acetate in mice in order to support the clinical demand in PET center.Methods By unloading the 11C-CO2 to the vessel which contained 0.2 ml CH3MgBr in THF,the first reaction could be going,then phosphoric acid(aqueous 17%v/v,0.2 ml)was added to the residue,and the produce was distilled at 170 ℃ into a vial containing 5 ml physiological phosphate buffer.The mice receiving 11C-acetate were sacrificed,the liver and tissue samples from other organs were weighed and radiocounted.Results Only 15 min was taken from 11C-CO2 to 11C-acetate and the yield was 34.6%.Chemical and radiochemical purity was more than 99% and 98%.The radioactivity was mainly distributed in liver and kidney.Conclusion The synthesis which has been improved the condition of reaction is rapid,effective and the yield is higher than before.更多还原