【摘要】 目的研究巨噬细胞移动抑制因子(MIF)在体外对大肠癌细胞侵袭能力的影响。方法大肠癌细胞株Lovo在外源性MIF刺激24h后用微孔迁移法检测通过微孔细胞数量的改变。RT-PCR检测基质金属蛋白酶2、9(MMP-2、MMP-9)和血管内皮生长因子(VEGF)mRNA表达情况。流式细胞术测定自发凋亡率变化。结果(1)MIF刺激后Lovo通过8μm微孔的细胞数增加[(174±9)个比(262±19)个,P=0.002)]。(2)VEGF121,VEGF165和MMP-9mRNA的表达增加(P<0.05)。MMP-2mRNA的表达无明显变化(P=0.616)。(3)Lovo自发凋亡率明显降低(P<0.001)。结论MIF能够抑制体外大肠癌细胞的凋亡,并能提高Lovo的体外侵袭能力。更多还原

【Abstract】 Objective To determine the influence of macrophage migration inhibitory factor(MIF)on the invasion of colorectal carcinoma cell(CC)lines,Lovo,in vitro.Methods The invasion of CC cell lines,Lovo,was evaluated by micron-migration assay in a chamber with 8 μm porosity polycarbonate filter membrane.RT-PCR was adopted to evaluate the mRNA level of matrix metalloproteinase-2,-9(MMP-2,-9)and vascular endothelial growth factor(VEGF).The change of apoptosis rate of CC cell lines was measured by flow cytometry.Results(1)The number of cells passing through filter membrane was significantly increased(174±9 vs 262±19,P=0.002).(2)VEGF121,VEGF165 and MMP-9 mRNA increased significantly(P<0.05).No apparent difference of MMP-2 mRNA expression(P=0.616)was observed.(3)The apoptosis rate of cells treated by MIF was reduced from 6.2%±0.45% to 2.2%±0.50%.Conclusions MIF can inhibit the apoptosis of CC cell and increase cell invasion of CC cell lines in vitro.Mild up-regulated MMP-9 and VEGF by MIF may be possibly involved in the invasion of CC cell lines.更多还原