【摘要】 目的:观察新生大鼠缺氧缺血性脑损伤后脑组织白细胞介素18的表达变化及其意义。方法:实验于2005-06/12在新乡医学院生理教研室和分子生物学实验室完成。选择7日龄SD大鼠112只,随机数字表法分为假手术组16只和缺氧缺血性脑损伤组96只,缺氧缺血性脑损伤组又分为术后3,8,24h,3,6,14d6个时相点,每个时相点16只。参照Rice等方法制备左脑缺氧缺血性脑损伤模型,采用westernblot的方法检测白细胞介素18在缺氧缺血性脑损伤后不同的时相点的表达变化,同时对脑组织进行苏木精-伊红染色,光镜下观察其病理变化。结果:纳入动物112只,均进入结果分析。①假手术组、缺氧缺血性脑损伤组术后3,8h白细胞介素18呈低水平表达,差异无显著性意义(分别为0.206±0.021,0.190±0.015,0.219±0.012,P>0.05)。与假手术组相比,缺氧缺血性脑损伤组术后24h白细胞介素18蛋白水平开始增加熏术后3,6d白细胞介素18蛋白水平继续增强,到术后14d达到高峰,差异均有显著性意义(分别为0.293±0.075,0.307±0.052,0.419±0.038,0.827±0.068,0.206±0.021,P<0.01)。②苏木精-伊红染色结果表明,神经元细胞变性坏死在缺氧缺血性脑损伤后1～6d逐渐加重,14d坏死区胶质细胞增生明显。结论:新生大鼠缺氧缺血脑损伤后白细胞介素18蛋白的表达增加,这一时期正发生着一系列的病理变化,提示白细胞介素18在缺氧缺血性脑损伤中发挥了促损伤作用。更多还原

【Abstract】 AIM: To observe the expression of interleukin (IL) -18 protein in brain tissue of hypoxia/ischemia neonatal rats and its significance. METHODS: The experiment was conducted at the Department of Physiology and Laboratory of Molecular Biology, Xinxiang Medical College between June and December 2005. A total of 112 neonatal SD rats, aged seven-day-old, were divided randomly into 2 groups: sham-operation group (n=16) and hypoxia/ischemia brain damage (HIBD) group (n=96). There were 6 time phases: 3, 8 and 24 hours, 3, 6 and 14 days with 16 rats in each group. HIBD models in left brain were established according to the methods presented by Rice et al. The expression of IL-18 protein was measured at different time phases by Western blot method. At the same time, the brain tissues were observed with hematoxylin-eosin (HE) staining and their pathological change was observed under light microscope. RESULTS: Totally 112 included rats entered the result analysis. The expression of IL-18 protein was low in sham-operation group and HIBD 3 and 8 hours group, and there was no significant difference (0.206±0.021,0.190±0.015,0.219±0.012,P > 0.05, respectively). Compared with the sham-operation group, the expression of IL-18 in damage brain tissue increased in the HIBD group from hour 24, increased progressively at days 3 and 6, and reached the peak at day 14, and there was significant difference (0.293±0.075,0.307±0.052,0.419±0.038,0.827±0.068,0.206±0.021,P < 0.01, respectively). ②HE staining showed that the degenerated and necrotic neurons increased progressively from day 1 to day 6 after HIBD, at day 14 the striking proliferation of glial cell appeared. CONCLUSION: The expression of IL-18 protein after HIBD increased in neonate rats, and a serial of pathological change appears in this phase. It is indicated that the IL-18 may play important roles in accelerating injury of HIBD in neonatal rats.更多还原