【摘要】 目的:观察壳多糖-胶原凝胶对口腔黏膜成纤维细胞生物学行为的影响。方法:实验于2003-10/2004-04在中国医科大学第二医院卫生部小儿先天畸形实验室和病毒室完成。①大鼠口腔黏膜成纤维细胞原代培养。②壳多糖-胶原凝胶制作:参照文献方法Plate1的配方在冰浴条件下,先后加入一定量的I型鼠尾胶原,两倍浓缩DMEM、胎牛血清、壳多糖,充分搅拌混匀,用0.1mol/LNaOH,调pH值至7.3左右,1h后凝固。③浸提液的制作:按体积分数为0.1的胎牛血清DMEM液,与壳多糖-胶原凝胶表面积之比为10mL/cm2加入培养液,于4℃下静置24h。④用噻唑蓝比色分析法,在光镜下观察、检测大鼠口腔黏膜成纤维细胞在壳多糖-胶原浸提液中和体积分数为0.1的胎牛血清DMEM液中生长、形态、增殖情况。结果:大鼠口腔黏膜成纤维细胞在壳多糖-胶原浸提液中能较长时间保持增殖活力,与含血清基础培养基中生长良好的大鼠口腔黏膜成纤维细胞,在细胞形态、吸光度值方面相似(P>0.05)。结论:壳多糖-胶原凝胶对口腔黏膜成纤维细胞的生长繁殖无抑制作用。更多还原

【Abstract】 AIM:To observe the effect of chitosan-collagen gels on the biological behavior of oral mucosa fibroblasts(OMFs).METHODS:The experiment was performed at the Children Congenital Deformity Laboratory and Virus Room of Department of Health,Second Hospital of China Medical University from October 2003 to April 2004.①OMFs of rats were cultured primarily.②Preparation of chitosan-collagen gels:Based on the Plate1 prescription in reference,I type collagen was added early or late,twice concentrated DMEM,fetal calf serum,chitosan,mixed evenly under iced bath condition.With 0.1 mol/L NaOH,pH value was adjusted to about 7.3,and conagulated 1 hour later.③Preparation of leaching liquor:According to 0.1 volume fraction DMEM liquor of fetal calf serum was moved into culture solution based on 10 mL/cm2 of chitosan-collagen gel at 4℃ for 24 hours.④Under light microscope growth,formation and proliferation of OMFs in chitosan-collagen gel leaching liquor and 0.1 volume fraction fetal calf serum with MTT microassay.RESULTS:OMFs of rats in chitosan-collagen gel leaching liquor could keep proliferation activity as well as those in serum containing medium,no significant difference in morphology and absorbance(A)(P > 0.05).CONCLUSION:Chitosan-collagen gels have no inhibitive effect on the proliferation of OMFs.更多还原