【摘要】 目的:通过测定糖耐量变化,分析荔枝核提取液对四氧嘧啶糖尿病模型小鼠生化指标的干预作用。方法:实验于2005-12/2006-01在右江民族医学院生化教研室完成。①荔枝核,为广西百色市靖西县产酸荔枝果核,晒干后粉碎,水提取,加热煮沸30min后,过滤去渣浓缩,加乙醇沉淀,再过滤去沉淀,加热挥发乙醇,提取,得到含浓度1.2g/mL生药水提液。②选取清洁级健康昆明种小白鼠40只,分为正常对照组、模型对照组、荔枝核提取液低浓度组、荔枝核提取液高浓度组,10只/组。③除正常对照组外,其余各组均按200mg/kg体质量腹腔注射四氧嘧啶水溶液建立糖尿病模型。48h后测血糖,血糖未升高的小鼠按100mg/kg腹腔注射四氧嘧啶水溶液,连续追加2次。③待空腹血糖升高和糖耐量异常后,荔枝核提取液低浓度组用荔枝核水提取液给予灌胃治疗,每只小鼠剂量为0.5mL(内含0.012g荔枝核生药);荔枝核提取液高浓度组每只小鼠剂量为0.5mL(内含0.024g荔枝核生药);正常对照组、模型对照组给予等量蒸馏水灌胃。1次/d,连续7d。④测定造模前后空腹血糖,治疗前后糖耐量血糖,治疗后血清丙氨酸氨基转移酶、尿素、三酰甘油、总胆固醇含量。制备大脑匀浆,测定乙酰胆碱酯酶活性、超氧阴离子自由基(O2-)浓度和清除率、蛋白含量。结果:实验选取健康昆明种小白鼠40只,全部进入结果分析。①造模前后各组小鼠血糖含量的变化:与造模前比较,造模后2,6,9d模型对照组、荔枝核提取液低浓度组、荔枝核提取液高浓度组血糖含量均显著升高,而正常组无明显变化。②治疗前后各组糖耐量试验血糖含量的比较:治疗前30min,与正常对照组比较,其余3组血糖含量均明显升高(P<0.05或P<0.01),显示耐糖量较差。治疗后30min,与正常对照组比较,其余3组血糖含量亦均明显升高(P<0.05或P<0.01)。治疗后2h除模型对照组外,荔枝核提取液低浓度组、荔枝核提取液高浓度组均恢复到治疗前空腹水平。③治疗后各组小鼠血清生化指标的测定结果:荔枝核提取液高浓度组总胆固醇明显高于荔枝核提取液低浓度组、正常对照组;荔枝核提取液高浓度组、荔枝核提取液低浓度组、模型对照组尿素含量均明显高于正常对照组(P<0.01)。④治疗后各组小鼠脑匀浆中生化指标的测定结果:模型对照组乙酰胆碱酯酶活性和O2-浓度均明显高于其他各组(P<0.01),O2-清除率明显低于其他各组(P<0.01),脑蛋白明显低于荔枝核提取液高浓度组(P<0.05)。结论:荔枝核提取液能够明显改善四氧嘧啶糖尿病小鼠大脑胆碱能系统传递功能,加速超氧阴离子自由基的清除率,对血脂和肝、肾功能的保护作用尚不明显。更多还原

【Abstract】 AIM: To explore the intervention of litchi nucleus extract fluid on blood biochemical indexes of mice models with diabetes mellitus induced by alloxan. METHODS: The experiment was carried out in the Biochemical Institute, Youjiang Medical College of Nationalities between December 2005 and January 2006. (1)The extract fluid containing 1.2 g/mL crude drug was prepared with the sour litchi nucleus from Jingxi County, Baise City of Guangxi, shattered after drying and extracted with water, followed by filtering and concentrating after 30 minutes of heating to precipitate with alcohol, and filtered again to remove the precipitate, and heated to volatilize the alcohol. (2)Forty clean healthy Kunming mice were selected and divided into 4 groups: Control group, model group, low concentration extract fluid group and high concentration extract fluid group with 10 mice in each group. (3)Except for the control group, all the rest mice were intraperitoneally injected with alloxan liquid to establish the diabetes models. The blood glucose was measured 48 hours later, those whose blood glucose was not increased were reinjected the alloxan for successive twice by body mass of 100 mg/kg. (4)When the fasting blood glucose (FBG) increased and the sugar tolerance was abnormal, the litchi nucleus extract fluid with dose of 0.5 mL containing 0.012 g and 0.024 crude drug was infused into the stomachs of mice of the low and high concentration groups, respectively; While the mice in the control and model groups were given the same dose of distilled water. The administration was given once a day for 7 days. (5)FBG before and after establishment of models, sugar tolerance before and after treatment, alanine aminotransferase (AAT) in blood, urea, triglyceride (TG) and total cholesterol (TC) after treatment were determined. The acetylcholine esterase (Ache) activity, density and clearance of superoxide radical anion (O2-) and the protein content were determined after preparation of homogenized brain. RESULTS: All the 40 mice were involved in the result analysis. (1)Changes of FBG before and after establishment of models: FBG of the low and high concentration groups on the 2nd, 6th and 9th day after establishment of models was increased compared with before establishment of models; while there was no obvious change in the control group. (2)Comparison of sugar tolerance before and after treatment: Blood glucose in the low and high concentration groups and the model group were significant higher than that in the control group at the point of 30 minutes before treatment (P < 0.01 or P < 0.05), indicating the sugar tolerance was worse. The same case appeared at the point of 30 minutes after treatment (P < 0.01 or P < 0.05). At the 2nd hour after treatment, the blood glucose of low and high concentration groups recovered to the level of FBG except for the control group. (3)The results of serum biochemical indexes after treatment: TC in the high concentration group was significantly higher than that in the low concentration and control groups; the serum urea in the low and high concentration groups and the model group were higher than that in the control group (P < 0.01). (4)The results of biochemical indexes in homogenized brain after treatment: AchE activity and density of O2- in the model group were higher than that in other three groups (P < 0.01), while the clearance were obviously lower than that in other three groups (P < 0.01); protein content of homogenized brain in the model group were significant lower than that in the high concentration group (P < 0.05). CONCLUSION: Litchi nucleus extract fluid could significantly improve the transmit function of cholinergic nerve system in cerebrum of mice with diabetes mellitus induced by alloxan, and accelerate the clearance of O2-, but has no significant protective effect on blood lipid, liver and kidney function.更多还原