【摘要】 目的:探讨新型磷酸钙骨水泥(BiopexR)骨内移植的生物学特点。方法:实验于2004-04/2005-04在吉林大学基础医学院动物室完成。①取4～6个月龄健康成熟中国大白兔18只,在双侧股骨内侧髁部建立骨缺损骨水泥填充模型。②将BiopexR(粉液比为3∶1),用专用注射器注入骨孔内并使骨水泥稍外溢,同时指压2min至骨水泥凝固。③通过光镜及电镜观察植入后3,6,12及24周时BiopexR的组织学改变。结果:①光镜观察:3周时,可见BiopexR同骨床直接愈合,没有纤维组织介入。BiopexR表面有新骨形成。6周时,BiopexR量开始减少,其表面新骨形成增多,同时可见新骨向BiopexR内部生长,新骨与BiopexR交织在一起。12周时,BiopexR量明显减少,新生骨小梁增粗,交织成网状。24周时,BiopexR量进一步减少,可见成熟骨细胞,出现板层骨。②透射电镜:观察:3周时,在BiopexR表面可见许多成骨细胞,细胞表面微小突起伸向BiopexR内部,成骨细胞胞浆内可见较多粗面内质网。同时可见一些破骨细胞。在BiopexR与骨床之间可见一透亮线,但并非是纤维组织,表明结合力较弱。12周时,BiopexR密度减低,呈网状结构。在BiopexR内部可见散在的成骨细胞,粗面内质网扩张,线粒体轻度空化。在BiopexR与骨床之间未见透亮线,表明已牢固结合。结论:BiopexR具有良好的生物相容性、骨传导性及可降解性,是一种理想的骨移植材料。更多还原

【Abstract】 AIM: To investigate the biological characters of the new calcium phosphate cement (BiopexR) as a bone graft material. METHODS: This experiment was conducted at the Animal Experimental Laboratory, College of Basic Medical Sciences, Jilin University from April 2004 to April 2005.①In 18 Chinese healthy adult rabbits of 4-6 months, the BiopexR was filled into the femoral condyiar defect of rabbits to establish animal models.② BiopexR (the ratio of power and solution was 3:1) was injected into the pore with specialized syringe and the bone cement overflowed the pore , and at the same time , press it for 2 minutes till the cement concreted . ③ Histological change of BiopexR was observed at week 3,6,12 and 24 after surgery under light microscope and electron microscope.RESULTS: ①Observation under the light microscope: at the 3rd week after surgery, new bone was formed on the surface of BiopexR, the new bone connected directly to the material surface,and there was no intervening fibrous tissue between the BiopexR and the host bone. At the 6th week after surgery, the amount of BiopexR began to decrease, the trabecula of the new bone could be seen on the surface of BiopexR. BiopexR and the new bone were interlaced, the new bone invaded in the BiopexR. At the 12th week after surgery, BiopexR decreased obviously, the trabecula of the new bone developed and the material and new bone interlaced together so as to form webs. At the 24th week after surgery, the amount of BiopexR decreased further, mature osteocytes and laminar bone could be seen. ②Observation under the transmission electron microscopy: At the 3rd week after surgery, many osteoblasts were found on the surface of the material, the protuberance of osteoblast invaded in the BiopexR, more rough endoplasmic reticulum (RER) were observed, and the osteoclasts could also be found. BiopexR and new bone were integrated directly, there was no fibrous tissue between the BiopexR and the new bone,but there was a light line between them, suggesting they were not firmed up. At the 12th week after surgery, the density of BiopexR decreased, presenting netted structure. Some scattered osteoblasts were found in the BiopexR, RER swelled, and there was the phenomenon of mitochondrion cavitation. BiopexR and new bone were integrated directly; there was not a light line between them. This showed they were firmed up.CONCLUSION: BiopexR has a good biocompatibility, good osteoconduction and capability of absorbency. BiopexR is a perfect bone graft material.更多还原