【摘要】 目的:观察手针和电针预处理对全脑缺血大鼠脑组织细胞凋亡的影响,并与缺血预处理结果进行比较。方法:①实验于2005-02/2005-9在黑龙江中医药大学实验中心完成。①选用雄性健康Wistar大鼠108只。采用4-动脉阻断法复制大鼠全脑缺血模型。按随机抽签法将大鼠分为6组(每组18只):正常组(正常饲养,不予任何处置),假手术组(仅暴露双侧椎动脉及双侧颈总动脉),脑缺血组(热凝双侧椎动脉及动脉夹夹闭双侧颈总动脉10min),脑缺血预处理组(热凝双侧椎动脉及动脉夹夹闭双侧颈总动脉3min,24h后全脑缺血10min),手针预处理组(术前7d用0.25mm×40mm毫针针刺大鼠双侧足三里穴,直刺进针5mm;双侧曲池穴,直刺进针4mm,0.25mm×25mm毫针针刺百会穴,平刺约3mm。针刺预处理组间歇5min捻转一次,平补平泻,1次/d,干预30min,预处理后24h全脑缺血10min),电针预处理组(术前7d采用全能脉冲电疗仪,对足三里、曲池穴进行电针针刺,频率为1Hz,电压为2V,强度以肢体轻微抖动为度,1次/d,干预30min。预处理后24h全脑缺血10min)。②术后24,48,72h各组各处死鼠6只,取脑,采用细胞凋亡原位末端标记法检测大鼠脑组织的细胞凋亡情况。③组间计量资料比较采用方差分析。结果:大鼠108只均进入结果分析。大鼠大脑皮质及海马CA1区细胞凋亡数:脑缺血预处理组、手针预处理组、电针预处理组、假手术组、正常组明显少于脑缺血组(P<0.05);电针预处理组大鼠大脑皮质细胞凋亡数明显少于手针预处理组(P<0.05),与脑缺血预处理组相近。结论:针刺预处理可以通过抑制神经细胞凋亡,且电针干预效果与脑缺血预处理相近,优于手针干预后。更多还原

【Abstract】 AIM: To investigate effect of hand acupuncture and electro-acupuncture pretreatment on apoptosis in brain tissue of rats with global cerebral ischemia and compare with the effect of ischemic pretreatment. METHODS: The experiment was completed at the Experimental Center of Heilongjiang University of Traditional Chinese Medicine from February to September 2005. ① Totally 108 male healthy Wistar rats were selected in this study. Models of global cerebral ischemia of rats were copied with four vessels occlusion. All rats were divided into 6 groups with 18 in each group according to random lot method. Rats in normal group were fed normally and not treated with any methods. Rats in sham operation group were exposured bilateral vertebral artery and bilateral common carotid artery. Bilateral vertebral artery of rats in cerebral ischemia group was thermocoagulated and bilateral common carotid artery was clipped with artery clamp for 10 minutes. Bilateral vertebral artery of rats in cerebral ischemia pretreatment group was thermocoagulated and bilateral common carotid artery was clipped with artery clamp for 3 minutes. Twenty-four hours later, global cerebral ischemia was maintained for 10 minutes. Rats in hand acupuncture treatment group were acupunctured at bilateral Zusanli vertically with milli-needle sized 0.25 mm × 40 mm in the depth of 5 mm 7 days before operation, at bilateral Quchi vertically in depth of 4 mm, and at Baihui flatly with milli-needle sized 0.25 mm × 25 mm in the depth of 3 mm. Needle was twirled once every 5 minutes, and uniform reinforcing-reducing method was interfered once a day for 30 minutes. Twenty-four hours after pretreatment, global cerebral ischemia was maintained for 10 minutes.Rats in electro-acupuncture pretreatment group were acupunctured at Zusanli and Quchi at frequency of 1 Hz and voltage of 2 V with omnipotent impulse electro-therapeutic apparatus 7 days before operation. Light tremble of limbs was regarded as the standard intensity. Acupuncture was interfered once a day for 30 minutes. Twenty-four hours after pretreatment, global cerebral ischemia was maintained for 10 minutes. ② Six rats in each group were sacrificed at time points of 24, 48 and 72 hours to obtain brain. Numbers of apoptosis in brain tissue were detected with in situ end-labeling (ISEL) method. ③ Measurement data were compared with analysis of variance. RESULTS: Totally 108 rats entered the final analysis. Numbers of apoptosis in cerebral cortex and hippocampal CA1 area: Numbers were less in cerebral ischemia pretreatment group, hand acupuncture pretreatment group, electro-acupuncture pretreatment group, sham operation group and normal group than those in cerebral ischmia group (P < 0.05); numbers in cerebral cortex were less in electro-acupuncture pretreatment group than those in hand acupuncture pretreatment group (P < 0.05), and was close to those in cerebral ischemia pretreatment group. CONCLUSION: Acupuncture pretreatment can inhibit apoptosis of nerve cells. Effect of electro-acupuncture is close to that of cerebral ischemia pretreatment and superior to that of hand acupuncture.更多还原