【摘要】 目的:观察苦豆子水煎剂对豚鼠离体内脏平滑肌作用的影响,分析其发挥作用途径。方法:实验于2004-12在宁夏医学院基础学院生理实验室完成。①选用健康成年三色毛豚鼠24只,雌雄不拘。②苦豆子(由银川市中医院提供,由该院检定)被制备成1kg/L的水煎剂,实验的系列质量浓度为1×10-4,3×10-4,1×10-3,3×10-3,1×10-2,2×10-2kg/L。③实验方法:实验时分别取出豚鼠胆囊、胃、小肠及膀胱。每个胆囊制备3条平滑肌肌条,共72条;胃、小肠及膀胱各制备12条平滑肌肌条。累积于置胆囊、胃、小肠及膀胱平滑肌肌条各12条的肌槽中加入1×10-4,3×10-4,1×10-3,3×10-3,1×10-2,2×10-2kg/L苦豆子水煎剂,加药间隔2min,容量50μL。④按随机抽签法将其余60条胆囊肌条分为5组:阿托品+苦豆子组、酚妥拉明+苦豆子组、维拉帕米+苦豆子组、苯海拉明+苦豆子组、吲哚美辛+苦豆子组,每组12条。上述5组分别加入拮抗剂阿托品(胆碱能M受体阻断剂)1×10-6mol/L,酚妥拉明(肾上腺素能α受体阻断剂)1×10-6mol/L,维拉帕米(Ca2+拮抗剂)1×10-7mol/L,苯海拉明(组织胺H1受体阻断剂)1×10-6mol/L,吲哚美辛(前列腺素受体阻断剂)1×10-6mol/L后2min再依次加入前述“累积加药”中各质量浓度苦豆子。⑤通过JH-2肌肉张力换能器将信号输出BL-410生物机能实验系统读出标本的张力、收缩波平均振幅及收缩频率的变化值。⑥数据间差异性比较采用均数t检验。结果:①累积加入苦豆子水煎剂1×10-4,3×10-4,1×10-3,3×10-3,1×10-2,2×10-2kg/L后,胆囊肌条张力逐渐增高、收缩频率逐渐加快、收缩波平均振幅逐渐减小。②当苦豆子水煎剂累积质量浓度为2×10-2kg/L时,酚妥拉明+苦豆子组离体胆囊肌条张力和收缩频率明显低于或慢于苦豆子组(t=2.3401,2.1404,P<0.05),而收缩波平均振幅明显高于苦豆子组(t=2.8256,P<0.05)。维拉帕米+苦豆子组离体胆囊肌条张力明显低于苦豆子组(t=2.3464,P<0.05)。③当苦豆子水煎剂累积质量浓度为1×10-2kg/L时,酚妥拉明+苦豆子组离体胆囊肌条张力明显低于苦豆子组(t=2.430,P<0.05),而收缩波平均振幅明显高于苦豆子组(t=2.2279,P<0.05)。维拉帕米+苦豆子组离体胆囊肌条张力明显低于苦豆子组(t=2.6576,P<0.05)。④累积加入不同质量浓度苦豆子水煎剂后对豚鼠胃、小肠及膀胱肌条的张力、收缩波平均振幅及收缩频率无明显影响(P>0.05)。结论:①苦豆子水煎剂可呈剂量依赖性增高胆囊平滑肌肌条的张力、加快收缩频率、减小收缩波平均振幅,其作用可被酚妥拉明及维拉帕米部分阻断,且该种作用可能与胆囊平滑肌细胞膜上的肾上腺素能α受体和细胞膜上的Ca2+通道有关。②苦豆子水煎剂对豚鼠胃、小肠及膀胱平滑肌的收缩活动无明显影响。更多还原

【Abstract】 AIM: To investigate effect and pathway of kudouzi decoction on smooth muscle of isolated splanchna in guinea pig. METHODS: The experiment was completed at the Physiological Laboratory of Basic College of Ningxia Medical College in December 2004. ① Totally 24 adult healthy guinea pigs of either gender with 3 colors of fur were selected in this study. ② Kudouzi provided by Yinchuan Hospital of Traditional Chinese Medicine was made into 1 kg/L decoction. Experimental mass concentrations were 1 × 104, 3×10-4, 1×10-3, 3×10-3, 1×10-2 and 2×10-2 kg/L. ③ Experimental method: Cholecyst, stomach, small intestine and bladder were obtained from guinea pigs. Three muscular strips of smooth muscles were established in each cholecyst with 72 in total, and 12 muscular strips were in stomach, small intestine and bladder, respectively. Muscular sockets of 12 muscular strips in cholecyst, stomach, small intestine and bladder were added with 1 × 104, 3×10-4, 1×10-3, 3×10-3, 1×10-2 and 2×10-2 kg/L kudouzi decoction, respectively. The interval was 2 minutes and the volume was 50 μL. ④ According to random lot method, 60 muscular strips were divided into 5 groups: atropine + kudouzi group, phenolamine + kudouzi group, verapamil + kudouzi group, aleryl + kudouzi group and indomethacin + kudouzi group, with 12 in each group. Guinea pigs in the above groups were treated with 1 × 10-6 mol/L atropine antagonist (blocking agent of cholinergic M receptor), 1×10-6 mol/L phenolamine (blocking agent of adnephrin α receptor), 1×10-7 mol/L verapamil (Ca2+ antagonist), 1×10-6 mol/L aleryl (blocking agent of histamine H1 receptor) and 1×10-6 mol/L indomethacin (blocking agent of prostaglandin receptor), and then kudouzi at above concentrations was added 2 minutes later. ⑤ Signals were output with JH-2 muscle tension converter; tension of samples, average amplitude of systolic wave and changes of systolic frequency were recorded with BL-410 biological function experimental system. ⑥ Differences of data were compared with mean t test. RESULTS: ① Tension of muscular strips in cholecyst was increased, systolic frequency was fastened, and average amplitude of systolic wave was shortened after adding with 1 × 104, 3×10-4, 1×10-3, 3×10-3, 1×10-2 and 2×10-2 kg/L kudouzi decoction. ② When mass concentration of kudouzi decoction was 2×10-2 kg/L, tension and systolic frequency of muscular strips of isolated cholecyst were less in phenolamine + kudouzi group than those in kudouzi group (t=2.340 1, 2.140 4, P < 0.05), but average amplitude of systolic wave was higher than that in kudouzi group (t=2.825 6, P < 0.05). Tension of muscular strips was weaker in verapamil + kudouzi group than that in kudouzi group (t=2.346 4, P < 0.05). ③ When mass concentration of kudouzi decoction was 1×10-2 kg/L, tension of muscular strips of isolated cholecyst was less in phenolamine + kudouzi group than that in kudouzi group (t=2.430, P < 0.05), but average amplitude of systolic wave was higher than that in kudouzi group (t=2.227 9, P < 0.05). Tension of muscular strips was weaker in verapamil + kudouzi group than that in kudouzi group (t=2.657 6, P < 0.05). ④ There was no effect of kudouzi decoction at various mass concentrations on tension of muscular strips, average amplitude of systolic wave and systolic frequency in stomach, small intestine and bladder of guinea pigs (P > 0.05). CONCLUSION: ① Kudouzi decoction has dosage-dependence on increasing tension of muscular strips, fastening systolic frequency and decreasing average amplitude of systolic wave. The effect can be blocked by phenolamine and verapamil partially, and the mechanism is possibly related with either adnephrin α receptor or Ca2+ channel of cell membrane of smooth muscle in cholecyst. ② There is no effect of kudouzi decoction on contraction of smooth muscle in stomach, small intestine and bladder of guinea pigs.更多还原