【摘要】 目的:探讨针刺预处理对全脑缺血大鼠海马CA1区B细胞淋巴瘤2mRNA表达的影响,并与缺血预处理的效果进行比较。方法:实验于2003-10在黑龙江中医药大学神经解剖实验室进行。取120只Wistar大鼠随机分为5组,每组24只:①正常对照组:不干预。②假手术组:暴露4条血管,不造模。③脑缺血组:四动脉阻断法全脑缺血10min制作大鼠全脑缺血模型。④脑缺血预处理组:预全脑缺血3min,再灌注24h后再次全脑缺血10min。⑤针刺预处理组:术前7d给予针刺,双侧足三里、曲池穴,双侧连接全能脉冲电疗仪,频率为1Hz,电压为2V,30min/次,针刺百会30min/次,1次/d,7d后全脑缺血10min。每组分别于再灌注12,24,48和72h麻醉状态下取材,免疫组织化学法检测海马CA1区B细胞淋巴瘤2蛋白阳性细胞数,原位分子杂交技术检测大鼠海马CA1区B细胞淋巴瘤2mRNA表达。结果:经补充后120只大鼠进入结果分析。①脑海马B细胞淋巴瘤2蛋白阳性细胞数:正常对照组和假手术组无;脑缺血组少量表达,再灌注48h为高峰,脑缺血预处理组和针刺预处理组24h、48h、72h均显著高于脑缺血组[(33.65±9.57),(34.56±12.64),(17.89±5.96)个/mm2;(39.14±9.11),(38.69±10.54),(23.35±7.68)个/mm2;(40.65±10.53),(38.99±9.34),(15.87±4.67)个/mm2;P<0.05],但两组间无差异(P>0.05)。②脑海马B细胞淋巴瘤2mRNA阳性细胞数:正常对照组和假手术组无;脑缺血组少量表达,再灌注72h为高峰,而脑缺血预处理组和针刺预处理组24h、48h、72h均显著高于脑缺血组[(42.64±9.57),(44.66±11.61),(20.8±5.97);(45.14±8.12),(46.68±11.54),(27.39±6.55);(50.65±10.53),(52.19±9.33),(20.87±6.67);P<0.05],但两组间无差异(P>0.05)。结论:针刺预处理可能通过上调B细胞淋巴瘤2mRNA和蛋白表达而减轻严重缺血后细胞凋亡并促成脑缺血耐受的产生。更多还原

【Abstract】 AIM To explore the effect of acupuncture precondition on B-cell lymphoma-2 mRNA expression in hippocampal CA1 area of rats with cerebral ischemia and compare with ischemic precondition. METHODS The experiment was completed at the Neurological Anatomy Laboratory of Heilongjiang University of Traditional Chinese Medicine in October 2003. Totally 120 Wistar rats were randomly divided into 5 groups with 24 in each group. ① Normal control group No interference. ②Sham-operation group Only 4 vessels were explored without modeling. ③ Cerebral ischemia group Rats were performed with cerebral ischemia for 10 minutes with 4-vessel-blocked method to establish models of cerebral ischemia. ④ Cerebral ischemic precondition group To block 4 blood vessels for 3 minutes first then 10 minutes after 24 hours. ⑤ Acupuncture precondition group Electroacupuncture on Zusanli and Quchi were completed 7 days before operation. Omnipotenc impulse electro-therapeutic apparatus frequency 1 Hz voltage 2 V 30 minutes/time was used to acupuncture Baihui at the speed of 30 minute/time once a day and then cerebral ischemia for 10 minutes 7days after operation. Samples were obtained under 12 24 48 and 72-hour reperfusion respectively. Positive neurons of B-cell lymphoma-2 in hippocampal CA1 area was counted with immunochistochemistry and B-cell lymphoma-2 mRNA expression was detected with molecular hybridization in situ. RESULTS Totally 120 rats entered the final analysis on the basis of supplement. ① Numbers of positive neurons of B-cell lymphoma-2 in hippocampus Positive neurons were not observed in both normal control group and sham-operation group a few of them were observed in cerebral ischemia group and the numbers reached at peak 48 hours after reperfusion. Positive neurons were increased at 24 hours 48 hours and 72 hours in ischemic precondition group and acupuncture precondition group as compared with those in cerebral ischemia group 33.65±9.57 34.56 ±12.64 17.89±5.96/mm2 39.14±9.11 38.69±10.54 23.35±7.68/mm2 40.65±10.53 38.99±9.34 15.87±4.67/mm2 P < 0.05 and there was no significant difference between two groups P > 0.05. ② Numbers of positive neurons of B-cell lymphoma-2 mRNA in hippocampus Positive neurons were not observed in both normal control group and sham-operation group a few of them were observed in cerebral ischemia group and the numbers reached at peak 72 hours after reperfusion. Positive neurons were increased at 24 hours 48 hours and 72 hours in ischemic precondition group and acupuncture precondition group as compared with those in cerebral ischemia group 42.64±9.57 44.66±11.61 20.8±5.97 45.14±8.12 46.68±11.54 27.39±6.55 50.65±10.53 52.19±9.33 20.87±6.67 P < 0.05 and there was no significant difference between two groups P > 0.05. CONCLUSION Acupuncture precondition can promote B-cell lymphoma-2 mRNA and protein expression to decrease apoptosis and protect cerebral ischemia injury.更多还原