【摘要】 目的:观察青少年女性中长跑运动员这一特殊群体在大运动量集训期前后血清细胞因子及生化指标的变化,探讨运动应激条件下运动员机体变化和细胞因子免疫网络的应答机制以及纯中药制剂--纳米力谷源胶囊对其的调节作用。方法:于2004-06选择北京市某体校青少年女性中长跑运动员20名,为运动员组。又分为运动员对照组和运动员服药组,每组10名。取自无免疫性疾病、无现症、体检健康的青少年女性10名,为正常对照组。运动员服药组服用纳米力谷源胶囊(北京长城制药厂生产,批号:20040201,所含成分为葛根素、精氨酸和玉米抗氧化活性物质),3次/d,2粒/次。两组运动员共同参加训练,使用相同的训练方案:训练时间2004-06-22/07-28,共5周。每日上午:20km×7次/周×5周=700km;下午:节奏跑10km×3次/周×5周=150km,随意跑40min×2次/周×5周=400min,其余两天下午休息。运动员组休整后于2004-06-21取第1次血,第2天开始5周集训,并于集训后2004-07-29取第2次血。应用EVIDENCE180全自动化学发光生物芯片分析仪和HITACHI7600型全自动生化分析仪对20名青少年女性中长跑运动员集训前后及服药后血液标本分别进行12项细胞因子(白细胞介素1α、白细胞介素1β、白细胞介素2、白细胞介素4、白细胞介素6、白细胞介素8、白细胞介素10、血管内皮生长因子、γ-干扰素、表皮生长因子、单核细胞趋化性肽1、肿瘤坏死因子α)和33项生化指标(三酰甘油、胆固醇、载脂蛋白A1、载脂蛋白B、高密度脂蛋白C、低密度脂蛋白C、谷丙转氨酶、谷草转氨酶、总蛋白、血清白蛋白、总胆红素、直接胆红素、总胆汁酸、钠、钾、氯、二氧化碳、钙、磷、镁、铁、不饱和铁结合力、总铁结合力、葡萄糖、尿素氮、肌酐、尿酸、肌酸激酶、乳酸脱氢酶、碱性磷酸酶、γ-谷氨酰转肽酶、淀粉酶、肌酸激酶同工酶)的定量检测,比较运动应激对机体的影响。结果:纳入运动员20名和正常对照青少年10名,均进入结果分析。①血清细胞因子检测结果:女性青少年长跑运动员在休整后安静状态下与正常人相比较表皮生长因子显著偏低[运动前、正常对照分别为(18.94±14.04),(62.97±38.07)ng/L];而运动员在一个时期的强度训练以后与运动前相比白细胞介素8显著下降[运动前后分别为(21.82±11.52)ng/L,<9.7ng/L],血管内皮生长因子也显著下降[运动前后分别为(113.13±47.34),(96.90±47.30)ng/L],表皮生长因子显著上升[运动前后分别为(18.94±14.04),(58.93±25.48)ng/L];运动员服药组在经过相同的训练期后与运动员对照组相比白细胞介素1β显著降低[运动员服药组、运动员对照组分别为(2.97±0.82),(3.90±0.53)ng/L],且接近正常人水平。②血清生化指标检测结果:女性青少年长跑运动员在一个时期的强度训练以后与运动前相比三酰甘油显著下降[运动前后分别为(0.74±0.21),(0.51±0.10)mmol/L]、胆固醇显著下降[运动前后分别为(4.29±0.83),(3.96±0.76)mmol/L]、高密度脂蛋白显著下降[运动前后分别为(1.60±0.24),(1.50±0.28)mmol/L]、血清白蛋白显著下降[运动前后分别为(45.77±1.57),(44.23±1.88)g/L]、葡萄糖显著下降[运动前后分别为(5.39±0.20),(4.85±0.30)mmol/L],尿酸显著上升[运动前后分别为(232.34±32.23),(278.89±22.69)μmol/L],可能与大运动量训练后造成机体贮存消耗有关,而钠、钾、氯显著下降,可能与出汗等生理反应有关。而运动员服药组服药后除三酰甘油显著上升[服药后、运动后分别为(0.89±0.41),(0.51±0.10)mmol/L]外,其他各项生化指标与运动员对照组相比变化不是很明显。结论:大运动量的集训可导致运动员机体应激性改变,免疫力下降,还可造成机体内贮能物质的大量消耗。纳米力谷源胶囊可有效的增强运动员机体的抗应激能力,提高运动员的免疫力和抗疲劳能力。更多还原

【Abstract】 AIM: To study the physical and immune function of female adolescent middle-distance race athletes in stage of fore-and-aft training and evaluate the adjusted effect of nanometer-particulate ligu yuan capsule, a traditional Chinese herb. METHODS: In June 2004, 20 female adolescent middle-distance race athletes were selected from a athletic school of Beijing and divided into two groups: control group and medication group with 10 athletes in each group. Ten healthy female adolescents were selected as normal control group. Athletes in medication group were treated with nanometer-particulate ligu yuan capsule with 3 times per-day and 2 capsules per-time. Athletes in the two groups were trained together with the same training plan: training period was from June 22, 2004 to July 28, 2004, total 5 weeks. They run 20 km every morning totally 700 km during 5 weeks, tempo run 10 km in the afternoon 3 times per-week totally 150 km during 5 weeks, random run 40 min in the afternoon 2 times per-week totally 400 minutes during 5 weeks, and the other two afternoon they could rest. All of 20 athletes were phlebotomized the first time at June 21, 2004 after a good rest period and phlebotomized the second time at July 29, 2004 after 5 weeks’ training. In order to evaluate the effect which caused by exercise hyperirritability, 12 different serum cytokines (interleukine-1α, interleukine-1β, interleukine-2, interleukine-4, interleukine-6, interleukine-8, interleukine-10, vascular endothelial growth factor, interferon γ, epidermal growth factor, monocyte chemoattractant protein-1, tumor necrosis factor α) and 33 different biochemical tests (triglyceride, cholesterol, apolipoprotein A1, apolipoprotein B, high density lipoprotein cholesterol, low density lipoprotein cholesterol, alanine aminotransferase, aspartate aminotransferase, total protein, albumin, total bilirubin, direct bilirubin, total bile acid, sodium, potassium, chloride, carbon dioxide, calcium, phosphorus, magnesium, iron, Unsaturated Iron Binding Capacity, Total Iron Binding Capacity, glucose, urea, creatinine, uric acid, creatine kinase, lactate dehydrogenase, alkaline phosphatase, γ-glutamyltransferase, amylase, creatine kinase isoenzyme MB) had been detected with EVIDENCE 180 fully automatic chemiluminescence biochips analyzer and HITACHI 7600 fully automatic analyzer. RESULTS: All of 20 athletes and 10 healthy adolescents entered the analysis of results. ① The results of serum cytokines: After a good rest period, compared with the normals, the serum epidermal growth factor (EGF) of female adolescent middle-distance race athletes decreased obviously (18.94±14.04) ng/L, (62.97±38.07 ng/L). Compared before and after training, the serum interleukine-8 (IL-8) of female adolescent middle-distance race athletes decreased obviously (21.82±11.52) ng/L, < 9.7 ng/L, vascular endothelial growth factor (VEGF) also decreased obviously (113.13±47.34) ng/L, (96.90±47.30) ng/L, EGF increased obviously (18.94±14.04) ng/L, (58.93±25.48) ng/L. After had the same training period, compared with athletes in medication group, the serum interleukine-1β (IL-1β) of control group decreased obviously control group: (2.97±0.82) ng/L; medication group: (3.90±0.53) ng/L and nearly approached to the level of normal control group. ② The results of biochemical tests: Compared before and after training, the triglyceride (TG) of female adolescent middle-distance race athletes decreased obviously (0.74±0.21) mmol/L, (0.51±0.10) mmol/L, cholesterol (CH) decreased obviously (4.29±0.83) mmol/L, (3.96±0.76) mmol/L, high density lipoprotein cholesterol (HDL-C) decreased obviously (1.60±0.24) mmol/L, (1.50±0.28) mmol/L, albumin (ALB) decreased obviously (45.77±1.57) g/L, (44.23±1.88) g/L, glucose (GLU) decreased obviously (5.39±0.20) mmol/L, (4.85±0.30) mmol/L, uric acid (Ua) increased obviously (232.34 ±32.23) μmol/L, (278.89±22.69) μmol/L, these maybe related to consume the energy of body, and sodium (Na), potassium (K), chloride (Cl), decreased obviously maybe caused by physiological responses such as sweat. But compared with medication group, after dosed nanometer-particulate ligu yuan capsule, there was only TG increased obviously (0.89±0.41) mmol/L, (0.51±0.10) mmol/L in the control group, the other results of biochemical tests had no statistical difference.CONCLUSION: Heavy intensity training can cause changes of irritability, decrease of immune-ability and consumption of stored energy. Nanometer-particulate ligu yuan capsule can improve anti-stress ability, immune capability and anti-fatigue ability of body.更多还原