【摘要】 目的:探讨中药银杏叶提取物杏丁注射液对豚鼠庆大霉素中毒性耳聋早期干预的作用。方法:①实验于2003-11-29/2004-01-05在泸州医学院附属医院川南听力与言语疾病防治中心完成。选用耳廓反射正常的健康杂色豚鼠24只。随机将鼠分为3组:庆大霉素模型组8只(16耳),庆大霉素+杏丁注射液组8只(16耳),对照组8只(15耳)。②庆大霉素模型组每天臀部肌注庆大霉素(西南药业股份有限公司)100mg/kg,连续7d;庆大霉素+杏丁注射液组每天臀部肌注庆大霉素100mg/kg+杏丁注射液(贵州益佰制药股份有限公司)0.4mL/kg,连续7d;对照组臀部肌注等量0.9%生理盐水7d。③于肌注药物3和7d后采用耳声发射仪进行测试,对1,2,4,6kHz的畸变产物耳声发射幅值进行比较分析。④计量资料差异比较采用t检验。结果:鼠24只(47耳)均进入结果分析。①庆大霉素模型组肌注庆大霉素3d后畸变产物耳声发射分析频率4,6kHz的幅值明显低于庆大霉素+杏丁组[(23.19±3.80),(26.40±4.70)dBSPL;(24.88±5.12),(29.96±5.74)dBSPL,t=2.079,3.763,P<0.05];分析频率为1和2kHz时两组耳声发射幅度差异不明显(P>0.05)。4个分析频率下庆大霉素+杏丁组与对照组耳声发射幅度差异不明显(P>0.05)。②庆大霉素模型组肌注庆大霉素7d后畸变产物耳声发射分析频率4,6kHz的幅值明显低于庆大霉素+杏丁组比较[(21.39±3.04),(24.83±3.27)dBSPL;(23.90±4.35),(27.90±4.15)dBSPL,t=2.239,4.269,P<0.05];分析频率为1和2kHz时两组耳声发射幅度差异不明显(P>0.05)。庆大霉素+杏丁组肌注庆大霉素+杏丁7d后畸变产物耳声发射分析频率2,4,6kHz的幅值明显低于对照组(t=3.127,2.121,2.352,P<0.05)。结论:杏丁在早期可有效拮抗庆大霉素对耳蜗毛细胞的损害。更多还原

【Abstract】 AIM: To investigate the early interventional effect of xingding solution extracted from ginkgo leaf on ototoxic deafness of guinea pigs induced by gentamycin. METHODS: ① The experiment was completed at Chuannan Audile and Language Disease Prevention and Cure Center Affiliated to Luzhou Medical College from November 11, 2003 to May 1, 2004. Totally 24 guinea pigs with normal distortion product otoacoustic emission (DPOAE) were randomly divided into 3 groups: gentamycin model group (n=8, 16 ears), gentamycin+ xingding solution group (n=8, 16 ears) and control group (n =8, 15 ears). ② Guinea pigs in gentamycin model group were injected with 100 mg/kg gentamycin (Southwest Pharmacological Limited Company) for 7 successive days. Guinea pigs in gentamycin+ xingding solution group were injected with 100 mg/kg gentamycin+0.4 mL/kg xingding solution (Guizhou Yibai Pharmacological Limited Company) for 7 successive days. Guinea pigs in control group were injected with 0.9% saline for 7 days. ③ DPOAE was monitored to observe the amplitude, and analyzed the change of amplitude of DPOAE at the frequencies of 1, 2, 4, 6 kHz among 3 groups 3 and 7 days after injection. ④ Measurement data were compared with t test. RESULTS: Totally 24 guinea pigs with 47 ears entered the final analysis. ① Amplitudes of DPOAE at 4 and 6 kHz in gentamycin model group were higher than those in gentamycin+ xingding solution group 3 days after injection (23.19±3.80), (26.40±4.70) dB SPL; (24.88±5.12), (29.96±5.74) dB SPL, t=2.079, 3.763, P < 0.05; but difference at 1 and 2 kHz between two groups was not significant (P > 0.05). Differences of amplitude at 4 frequencies between gentamycin+ xingding solution group and control group were not significant (P < 0.05). ② Amplitudes of DPOAE at 4 and 6 kHz in gentamycin model group were lower than those in gentamycin+ xingding solution group 7 days after injection (21.39±3.04), (24.83±3.27) dB SPL; (23.90±4.35), (27.90±4.15) dB SPL, t=2.239, 4.269, P < 0.05; but difference at 1 and 2 kHz between two groups was not significant (P > 0.05). Amplitudes at 2, 4 and 6 kHz were lower in gentamycin+ xingding solution group than those in control group 7 days after injection (t=3.127, 2.121, 2.352, P < 0.05). CONCLUSION: Xingding can inhibit damage of gentamycin from cochlear hair cell during early period.更多还原