【摘要】 以DL-2-氨基-Δ2-噻唑啉-4-羧酸(DL-ATC)为转化底物,采用来自假单胞菌(Pseudomonas sp.)TS1138的L-半胱氨酸合成酶系对DL-ATC进行生物转化合成L-半胱氨酸。对转化条件进行了研究,得出TS1138菌株合成L-半胱氨酸的最适转化条件;反应温度为42~44℃,转化时间为2.5 h,底物质量浓度为6 g/L,酶源细胞浓缩倍数为5倍。动力学研究表明,TS1138菌株L-半胱氨酸合成酶系的米氏常数(Km)为7.1 997 mmol/L,最大初反应速度Vmax=41.6629 mmol/(L.min)。更多还原

【Abstract】 L-cysteine was produced from DL-2-aminoΔ²-thiazoline-4-carboxylic acid（DL-ATC） by means of microbial transformation in the presence of L-cysteine synthesizing enzyme from Pseudomonas sp.TS1138.Conditions for bio-transformation were studied,and the optimum conditions were as follows,transformation temperature was 42-44℃,transformation time was 2.5 h,mass concentration of DL-ATC was 6 g/L,concentration times of enzyme was 5.Kinetic studies showed that Michaelis constant（K_m） of Lcysteine synthesizing enzyme from Pseudomonas sp.TS1138 is 7.1997 mmol/L and maximal reaction rate(V_max) is 41.6629 mmol/（L·min）.更多还原