【摘要】 目的:从细胞和分子水平观察隔药饼灸对兔高脂血症(HLP)合并动脉粥样硬化(AS)的作用机理。方法:将75只兔随机分为5组,即空白组、模型组、直接灸组、隔药饼灸组、药物(血脂康)组,每组15只。采用免疫反应性损伤法建立兔HLP合并AS模型。穴位选取“巨阙”“天枢”“心俞”“肝俞”等,每穴30min,每日1次,连续治疗6周。聚合酶链式反应(RT-PCR)法测定主动脉血管细胞粘附分子-1(VCAM-1)的基因表达。结果:隔药饼灸组、直接灸组及药物组均使兔HLP合并AS主动脉VCAM-1的mRNA表达显著下调(P<0·01)。结论:隔药饼灸对兔HLP合并AS的主动脉血管细胞有一定的保护作用。更多还原

【Abstract】 Objective: To observe the effect of herbal-cake-separated moxibustion on the expression of vascular cell adhesion molecule (VCAM)-1 in hyperlipemia (HLP) rabbits with concomitant aterosclerosis (AS) so as to explore the mechanism of moxibustion in improving HLP. Methods: Seventy-five New Zealand species rabbits were divided into control (n=13), model (n=14), direct-moxibustion (n=14), herbal-cake-separated moxibustion (n=14), and medication (“Xuezhikang”, a herbal drug for lowering blood lipid, n=13) groups. HLP with AS model was established by using immunologic injury method and high-fat forage feeding. Moxibustion was applied to “Juque” (CV 14), “Tianshu” (ST 25), “Xinshu” (BL 15), “Ganshu”(BL 18), etc. 30 min every acupoint, once daily, continuously for 6 weeks. The expression of VCAM-1 mRNA in the aorta wall was assayed with reversed transcription polymerase chain reaction (RT-PCR) technique. Results: Compared with control group, the expression of VCAM-1 mRNA in model group was up-regulated significantly(P<0.01); while in comparison with model group, VCAM-1 mRNA expression in herbal-cake-separated moxibustion, direct-moxibustion and medication groups was down-regulated obviously(P<0.01), suggesting a favorable regulation of the 3 methods on VCAM-1 mRNA expression. No significant difference was found among the 3 treatment groups (P>0.05). Conclusion: Moxibustion can down-regulate HLP-induced enhancement of VCAM-1 mRNA expression, which likely contributes to its effect in delaying the development of aortic endothelial AS in rabbits with HLP combined with AS.更多还原