【摘要】 探讨不同条件下在氧化低密度脂蛋白(Ox-LDL)对U937源巨噬细胞活性氧/活性氮(ROS/RNS)动力学变化的影响。通过观察Ox-LDL急性和慢性作用下细胞内一氧化氮(NO)、超氧阴离子(O2-·)和ROS等动力学变化,发现Ox-LDL都能显著诱导细胞内NO和O2-·的升高,且在急性刺激时NO的升高显著高于O2-·。此外,还观察到12h时ROS显著升高(P<0.01),而在24h时却明显下降,但仍显著高于对照细胞(P<0.01)。由此表明,在早期泡细胞形成的不同时间,调节相应自由基的代谢有助于减轻早期动脉粥样硬化的炎症反应。更多还原

【Abstract】 Effect of Ox-LDL on kinetic changes of ROS/RNS in U937-derived macrophages was investigated. In the present study, detecting dynamics of intracellular NO, O2·- and ROS under the acute and chronic exposure of U937-derived macrophages to Ox-LDL, we found that Ox-LDL significantly induced the increase of intracellular NO and O2·- in time-dependent manner, and that increase of intracellular NO was significantly higher than of O2·- in the acute exposure. Moreover, in chronic exposure, Ox-LDL significant induced the increase of ROS at 12 h, but at 24 h ROS levels significantly decreased than at 12 h (P<0.01) and significantly higher than control cells (P<0.01). The findings suggest that modulation of free radicals metabolism may be a potential therapeutic strategy against atherosclerotic inflammation in different phases of early foam cells.更多还原