【摘要】 RNA干扰(RNAi),即由双链RNA介导的转录后基因沉默的现象,已成为分析原生动物基因功能的有效手段。现对应用RNAi研究原生动物微管蛋白的功能和基体组装、纤毛虫大核基因组重排、纤毛虫刺丝泡的发生和作用、端粒酶及细胞周期蛋白的功能等方面进行综述。更多还原

【Abstract】 RNA interference (RNAi), triggered by double stranded RNA (dsRNA), was the post-transcrip-tional gene silencing (PTGS) based on sequence-specific degradation of mRNA. It had been proved to be an effi-cient method for analyzing gene functions in protozoa. In this paper, we reviewed the RNAi methods and their mainresearch progress in cell and molecular biology of protozoa which focused on the assembly of base body andfunctions of tubulins, cyclins and telomerase, gene rearrangement and exocytosis of the trichocyst of the ciliatedprotozoa.更多还原