【摘要】 由核基因编码的叶绿体前体蛋白能否正确定向和转运到叶绿体,对叶绿体的功能十分重要。采用酵母双杂交方法,筛选豌豆的cDNA表达文库,分析检测与Rubisco小亚基前体蛋白(preSSU)相互作用并参与其调控的蛋白。结果表明,共有6种不同类型的蛋白与preSSU互作,分别为组氨酸蛋白激酶基因、转运蛋白复合体、转录和翻译相关蛋白及复合体、信号传导相关基因和光合合成相关基因。通过与相关蛋白的相互作用,preSSU也许参与了植物抗逆信号传导系统,并在植物的生长发育、成熟和衰老过程中起重要作用。更多还原

【Abstract】 Correct,efficient targeting and translocation of the precursor proteins synthesized in the nucleus into chloroplast is vital for the chloroplast function.The yeast two-hybrid system was employed to screen a pea cDNA expression library,and investigate the factors involved in interaction with preSSU(precursor of small subunit of Rubisco).The protein product of six different kinds of genes were putative partners of preSSU: the candidate histidine protein kinase gene,the translocation protein complex,transcriptional and translocation related genes and proteins,plant defensin,the gibberrellin regulation factor and EREB transcriptional factor,and photosynthesis related genes.The preSSU probably participates in different processes involved in plant responses to stress and pathogen infection,and plays an important role in plant growth,maturity and senescence.However,further research is needed to determine how these factors interact with preSSU and regulate its import.更多还原