【摘要】 目的探讨艾滋病病毒(HIV)抗体检测确认实验免疫印迹试验(WB)是否能够有效区别筛查阳性的可疑标本和阴性标本。方法筛查实验采用酶联免疫吸附试验(ELISA),确认实验采用WB法;采用两样本含量较大时均数u检验方法分析阳性-可疑组及阳性-阴性组的S/CO比值,判断两组是否存在显著性差异。结果分析了92份阳性-可疑标本和63份阳性-阴性标本的S/CO比值,两组之间无显著性差异。结论研究和实践都表明,确认实验WB方法不能有效区别筛查阳性的可疑标本和阴性标本。WB方法在确定可疑标本方面存在相当大的不足。更多还原

【Abstract】 Objective To study whether confirmation test of HIV antibody(Western blot)was capable of effectively differentiating ’suspected’and ’negative’specimens that were all ’positive’in primary screening test.Methods Primary screening test applied ELISA assay;confirmation test applied Western blot assay.We applied statistic analysis(u-test)to judge whether it was significantly different in S/CO ratios between ’positive-suspected’and ’positive-negative’specimens.Results There were 92 ’positive suspected’specimens and 63 ’positive-negative’specimens.It was not significantly different between these two groups.Conclusion Our study and practice indicated that confirmation test of HIV antibody(Western blot)could not effectively differentiate ’suspected’and ’negative’specimens that were all ’positive’in primary screening test.WB assay had serious limitation in confirming ’suspected’ specimens.更多还原