【摘要】 研究了大肠杆菌细胞经超声处理后细胞膜性能的变化。采用二乙酸荧光素(FDA)、罗丹明123(Rh123)、二氯荧光黄双乙酸盐(DCFH-DA)、钙离子荧光剂Fura-2/AM染色,通过荧光分光光度计检测了在各种荧光强度下大肠杆菌细胞经300 W超声处理后细胞膜通透性、细胞膜电位、胞内活性氧、胞内钙离子的变化,再利用透射电镜观察了细胞状态。结果表明,大肠杆菌细胞经300 W超声处理90 s内对FDA、Rh123都逐渐降低,在90 s时,膜电位降低26.7%,膜通透性增加33.3%,90 s内ROS增加,并促进了Ca2+离子通道打开,Ca2+离子内流,同时大肠杆菌结构完整,内外膜间变模糊。更多还原

【Abstract】 This paper studiedthe function ofE.colimembrane after ultrasoundtreating.Thealterations of the fluorescent intensity of fluoresein diacetate(FDA),rhodamine 123(Rh123),2,7-Dichlorofluorescin diacetate(DCFH-DA) and Fura-2-pentakis(acetoxymethy)(FURA-2) was assayed by fluorescence spectrophotometer,fluorescent intensities were des-ignated as the alterations of membrane permeability,membrane potential,reactive oxygenspecies(ROS) andintracellular Ca2 +onE.coliinduced by ultrasound field on 300 W.Andthen theE.coliwas assayed by transmission electron microscope.The resulting showed thatthe fluorescent intensities of Rh123 and FDA were decreasedin a ti me-depended manner.Af-ter treated for 90 s,membrane potential decreased 26.8 % but membrane permeability in-creased 33.3 %,andintracellular ROS was increased so theincreasing of ROSlevel couldfa-cilitate the opening of Ca2 +channels.E.coil′s structure was intact,butE.coilwall,cyto-plasmdemarcationline and the lipid bilayer were clear.更多还原