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山羊-绵羊种间体细胞核移植重构胚构建

Goat-Sheep heterogeneous nuclear transplantation

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【作者】 赛务加甫张立杨丽彭新荣郭志林李向臣权富生安志兴郑月茂赵雪张涌

【Author】 SAI Wujiafu~(1,2),ZHANG Li~(1,3),YANG Li~1,PENG Xin-rong~1,GUO Zhi-lin~1, Ll Xiang-chen~1,QUAN Fu-sheng~1,AN Zhi-xing~1,ZHENG Yue-mao~1,ZHAO Xue~1,ZHANG Yong~1 (1 Institute of Bio-Engineering,Northwest A & F University,Yangling,Shaanxi 712100,China; 2 College of Animal Science and Technology,Xinjiang Shihezhi University,Shihezi,Xinjiang 832003,China; 3 Inner Mongolia Livestock Improvement Station,Huhhot,Inner Mongolia 010011,China)

【机构】 西北农林科技大学生物工程研究所西北农林科技大学生物工程研究所 陕西杨凌712100新疆石河子大学动物科技学院新疆石河子832003陕西杨凌712100内蒙古家畜改良工作站内蒙古呼和浩特010011

【摘要】 以山羊皮肤成纤维细胞为供体细胞,绵羊去核卵母细胞为受体细胞进行种间体细胞核移植,构建山羊-绵羊重构胚,并对其采用离子霉素联合6-DM AP法和胞质注射绵羊精子提取物法进行激活,比较两种不同激活方法对山羊-绵羊种间体细胞核移植重构胚的激活效果。结果表明,共构建获得山羊-绵羊重构胚238枚,重构胚在体外能够发育至囊胚阶段;离子霉素联合6-DM AP法激活的卵裂率为58.33%(70/120),囊胚发育率为4.28%(3/70);胞质注射绵羊精子提取物法激活的卵裂率为63.55%(75/118),囊胚发育率为6.66%(5/75),两种方法的激活效果差异不显著。

【Abstract】 Totally 238 heterogeneous embryos were reconstructed with goat skin fibroblasts as the donor cells and sheep enucleated oocytes as the recipient,and the effects of two activation methods(Ionomycin combined with 6-DMAP or injection of sperm extract) on the development of heterogeneous reconstructed embryos were compared.The results indicated that a total of 238 heterogeneous embryos were reconstructed,and sheep cytoplast supported mitotic cleavage of goat karyoplasts,and was capable of reprogramming the nucleus to achieve blastocyst stage embryos.The reconstructed embryos activated by Ionomycin combined with 6-DAMP reached 58.33%(70/120) oocyte cleavage rate and 4.28%(3/70) blastocysts rate,while activated by sperm extract reached 63.55%(75/118) oocyte cleavage rate and 6.66%((5/75)) blastocysts development rate.

【基金】 国家“863”高技术研究发展计划项目(2001AA213081)
  • 【文献出处】 西北农林科技大学学报(自然科学版) ,Journal of Northwest Sci-Tech University of Agriculture and Forestry(Natural Science Edition) , 编辑部邮箱 ,2006年09期
  • 【分类号】Q813.2
  • 【被引频次】2
  • 【下载频次】103
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