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水稻空间诱变稻瘟病抗性变异研究及抗性变异基因的分子标记

Study on resistance variation of space-induced lines to rice blast and molecular marker of the resistant mutant gene

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【作者】 洪彦彬杨祁云林佩珍王慧陈志强

【Author】 HONG Yan-bin~(1,2),YANG Qi-yun~1,LIN Pei-zhen~1,WANG Hui~2,CHEN Zhi-qiang~2(1 Plant Protection Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou,Guangdong 510640,China;2 College of Agriculture,South China Agriculture University,Guangzhou,Guangdong 510642,China)

【机构】 广东省农业科学院植物保护研究所华南农业大学农学院华南农业大学农学院 广东广州510640广东广州510642广东广州510640

【摘要】 对空间诱变的丽江新团黑谷SP1~SP3代株系稻瘟病抗性变异进行了分析。结果表明,经过空间诱变后,510株SP1代植株中有70株对接种的GD 531菌株由感病变为抗病,占总株数的13.7%;SP2代多数抗病株系对接种菌株的抗感分离比例符合3∶1或15∶1的规律,说明SP2代多数抗病株系受1对或2对显性主效基因控制;SP3代受1对抗病主效基因控制的抗病株系抗性继续分离,而受2对抗病主效基因控制的抗病株系抗性基本稳定;利用LR 8-SP2群体对丽江新团黑谷经过空间诱变后产生的抗病基因进行分子标记,结果发现该基因位于第9染色体上,与微卫星标记RM 409连锁。

【Abstract】 The research data on the variation of the resistance to rice blast in the SP1SP3 of lijiangxintuanheigu which was susceptible to blast after space-induction showed that 13.7% mutant plants in the SP1 were resistant to rice blast.In the SP2 rice space-induced lines,most of the resistant lines had a ratio of (3∶1) or 15∶1 of the deviation of resistance and susceptibility,implying the resistance was controlled by 1 pair or 2 pairs of major genes.In the SP3 rice space-induced lines,a part of lines whose resistance were controlled by a pair of major genes continued to demonstrate deviation of resistance,but for the lines whose resistance were controlled by 2 pairs of major genes,the resistance generally became stable.By using the LR8-SP2 population for the molecular markers of the blast resistant genes induced through space mutation,it was found that the resistant gene was in the 9th chromosome and was linked with the SSR marker RM409.

【基金】 国家“863”高技术发展计划与研究项目(2002AA241011);广东省自然科学基金项目(031994);广东省科技计划项目(2003C60404)
  • 【文献出处】 西北农林科技大学学报(自然科学版) ,Journal of Northwest Sci-Tech University of Agriculture and Forestry(Natural Science Edition) , 编辑部邮箱 ,2006年04期
  • 【分类号】S511
  • 【被引频次】19
  • 【下载频次】257
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