【摘要】 以4个马铃薯栽培品种为试材,进行了其茎段的离体再生研究。结果表明,东农303、夏波帝、鄂1 号茎段愈伤组织最佳诱导培养基为MS+6-BA 3．0 mg／L+NAA 0．01 mg／L;费乌瑞它为MS+6-BA 3．0 mg／L+ NAA 0．1mg／L,愈伤组织的诱导率均可达100％。费乌瑞它和鄂1号不定芽分化诱导的最佳培养基均为MS+ 6-BA 2．0 mg／L+NAA 0．2 mg／L;东农303为MS+6-BA 2．0 mg／L+NAA 0．1 mg／L:夏波帝为MS+6-BA 2．0 mg／L+NAA 0．3 mg／L,其不定芽分化率分别达96．7％．96．7％,83．3％和80．0％。各品种不定芽的诱导生根率均为100％,试管苗移栽成活率为95％。更多还原

【Abstract】 Four potato cultivars were regenerated in vitro from stem explants. The results showed that the optimal medium for callus induction was found to be MS + 6-BA 3. 0 mg/L + NAA 0. 01 mg/L for cv. Dongnong 303,cv. Shepody and cv. E No. 1, MS + 6-BA 3. 0 mg/L + NAA 0. 1 mg/L for cv. Favorite. The callus induction rate on those media reached up to 100%. The optimal medium for adventitious bud differentiation from stem callus was found to he MS+6-BA 2. 0 mg/L + NAA 0. 2 mg/L for cv. Favorite and cv. E No. 1,MS+6-BA 2. 0 mg/L + NAA 0. 1 mg/L for cv. Dongnong 303,and MS + 6-BA 2. 0 mg/L + NAA 0. 3 mg/L for cv. Shepody. The adventitious hud differentiation rate reached 96. 7%, 96. 7%, 83. 3% and 80. 0% for the four cultivars respectively,and the rooting rate was 100% for all cultivars. The survival rate of tube plantlets after transplantation reached 95%.更多还原