【摘要】 以己烯雌酚(DES)、琥珀酸酐和牛血清白蛋白(BSA)等为主要原料,采用混合酸酐反应(氯甲酸异丁酯法)将半抗原己烯雌酚与载体蛋白BSA联接制备成人工免疫原。用此免疫原免疫家兔,并以间接ELISA法测定抗体效价。结果表明,人工合成的抗原具有很强的免疫原性,被免疫兔血清中的抗DES抗体效价高达28。本试验为下一步制备抗DES单克隆抗体及研制快速检测DES残留的免疫试剂盒奠定了基础。更多还原

【Abstract】 Using diethylstilbestrol(DES),succinic anhydride and bovine serum albumin(BSA) as main stuff,the artificial-immunogen has been obtained by the mixed acid anhydride reaction(isobutyl chloroformate method) to connect hapten DES with carrier-protein BSA.Six female rabbits were immunized subcutaneously with this artificial-immunogen,the serum value of antibody were measured with indirect ELISA test.Animal immune test demonstrated that there were antibody against DES in rabbits serum,and the value of the antibody was 2~8.This study laid the basis of further research on the immune test kit for the DES remnant and Monoclonal antibody against the DES.更多还原