【摘要】 根据发表的鹅细小病毒GPV B株全基因组设计一对引物,采用PCR方法扩增出鹅细小病毒扬州株(GPV YZ)非结构蛋白基因NS1,克隆到植物表达载体pBI121中,转化至大肠杆菌感受态细胞JM109。经菌液PCR、双酶切及质粒PCR鉴定后,将阳性重组质粒转化感受态农杆菌LBA4404。经菌液PCR及质粒PCR分析,获得了真核表达载体pBI121-NS1,该载体的构建为进一步研究鹅细小病毒NS1基因的分子生物学特性打下良好基础。更多还原

【Abstract】 A pair of primers was designed according to the sequences published by GenBank in order to amplify NS1 gene of goose parvovirus Yangzhou strains(GPV YZ).The NS1 gene was obtained by PCR,and cloned into the pBI121 vector,and transformed into the E.coli component JM109.The expressing vector was identified by PCR and enzyme digestion and then transformed into agrobacterium tumefaciens LBA4404.After the identification by PCR from LBA4404 and pBI121-NS1 vector,the specific expression vector of GPV NS1 was constructed successfully.The construction of recombinant pBI121-NS1 plant expression vector provides a basis for the research on biological functions and applications of GPV NS1.更多还原