【摘要】 运用离子交换层析法、凝胶过滤层析法和密度梯度离心法对已作初步处理的RHDV分别进行提纯,并通过血凝性鉴定、抗原蛋白含量检测、ELISA和SDS-PAGE凝胶电泳等方法检测其纯度。结果表明,通过Sephadex-200凝胶过滤层析法所提纯病毒的血凝性和蛋白浓度最高;稀释至相同的蛋白浓度之后,该法所提纯的病毒ELISA检测的OD值最大;SDS-PAGE电泳条带1条,经免疫转印分析证明,该条带为RHDV蛋白特异性条带。表明通过该法提纯的病毒,回收率最高、蛋白最纯。更多还原

【Abstract】 Purifying the RHDV which has already been purified firstly by methods of ion exchange chromatography,gel chromatography,density gradient ultracentrifugation and examining the content of RHDV by analyzing HA and protein degree,examining the pureness by the test of ELISA and SDS-PAGE.The result showed that the RHDV purified by gel chromatography has the biggest HA,highest protein degree;The RHDV which is purified by this method showed one protein band on the electrophoresis and the biggest OD of ELISA when it was diluted to the same degree of protein in comparison of the other two methods,so it means that the RHDV purified by gel chromatography is more and purer.更多还原