【摘要】 目的:研究人脂多糖应答基因(Lrg)这一新分子在正常胎儿组织中的表达分布情况。方法:利用课题组制备的兔抗人Lrg免疫血清,对胎儿心脏、肝脏、肺脏、肾脏、胃、小肠、睾丸和皮肤等进行免疫组化染色;特异性标记lrg目的基因,用D igoxin标记检测试剂盒,对胎儿小肠及睾丸组织进行原位杂交检测。结果:免疫组化实验表明Lrg在几种检测组织内均有表达,表达水平由强到弱依次为胃、睾丸、食管、小肠、肝、肾、心、肺等;原位杂交方法检测表明人lrg-mRNA在睾丸及小肠组织内高表达。结论:人Lrg在正常胎儿组织中有表达,但分布情况有差异,可能是一种新型功能蛋白。更多还原

【Abstract】 Objective:To observe the distribution of human lipopolysaccharide respond gene(Lrg) in normal fetal tissues.Methods: The expression level of human Lrg in several normal fetal tissues was identified by(immunohistochemistry);Then the coding region of human lrg cDNA was labeled and used to probe human tissues to detect the expression level of human lrg-mRNA.Results: Immunohistochemistry assay showed that human Lrg was(expressed) in almost all tissues detected.The order of positive signal strength was stomach,testicle,esophagus,small intestine,liver,kidney,heart and lung;In situ hybridization results demonstrated that human lrg-mRNA was(expressed) highly in testicle and small intestine.Conclusion:The human Lrg protein may be a new functional(protein) and it can be discovered in most normal human fetal tissues.更多还原