节点文献
人外周血CD4~+ CD25~+调节性T细胞的分离、鉴定和功能特征
Isolation,identification and functional characterization of human CD4~+ CD25~+ Treg cells from human peripheral blood
【摘要】 目的:分离人外周血CD4+CD25+Treg细胞,并检测其功能。方法:RT-PCR技术检测CD4+CD25+Treg细胞中Foxp3的mRNA表达;与CD8+T细胞和CD4+CD25-T细胞共同培养,或加入外源性IL-2及IL-4,检测其抑制功能;流式细胞术检测IFN-γ、IL-4和IL-10。结果:CD4+CD25+Treg细胞高表达Foxp3,主要分泌IL-10,能够抑制CD8+T细胞和CD4+CD25-T细胞的增殖,高浓度IL-2能够阻断CD4+CD25+Treg细胞的抑制功能。结论:CD4+CD25+Treg细胞是一群具有免疫抑制功能的调节性T细胞,这种抑制作用能够被高浓度IL-2阻断。
【Abstract】 AIM: To isolate CD4~+ CD25~+ Treg cells from human peripheral blood, and study their functional characteristics. METHODS: The expressions of Foxp3 in human CD4~+ CD25~+ Treg cells were test by RT-PCR. The regulatory properties of CD4~+ CD25~+ Treg cells were assessed by co-culturing with CD4~+ CD25~- T cells and CD8~+ T cells, or adding exogenous IL-2. The detection of intracellular cytokine production of IL-4, IL-10 and IFN-γ was done by flow cytometry. RESULTS: CD4~+ CD25~+ Treg cells highly expressed Foxp3 and mainly synthesized IL-10 that suppressed the proliferation of CD4~+ CD25~- T and CD8~+ T cells. Its suppressive function was reversed by high concentration of IL-2 and (or) IL-4. CONCLUSION: CD4~+ CD25~+ Treg cells exhibited a subpopulation of immunoregulatory T cells with suppressive function, which could be reversed by high concentration of IL-2.
【Key words】 CD4~+ CD25~+ Treg cell; immune suppression; IL-2;
- 【文献出处】 细胞与分子免疫学杂志 ,Chinese Journal of Cellular and Molecular Immunology , 编辑部邮箱 ,2006年02期
- 【分类号】R392.1
- 【被引频次】32
- 【下载频次】1571