节点文献

人外周血CD4~+ CD25~+调节性T细胞的分离、鉴定和功能特征

Isolation,identification and functional characterization of human CD4~+ CD25~+ Treg cells from human peripheral blood

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 杨江华张永祥王芳喻荣彬苏川孙南雄

【Author】 YANG Jiang-hua~1, ZHANG Yong-xiang~1, WANG Fang~2, YU Rong-bin~3, SU Chuan~ 4* , SUN Nan-xiong~1~1Department of Infectious Diseases, the First Affiliated Hospital, ~2Department of Immunology, ~3School of Public Health, ~4Department of Pathogen Biology & Immunology, Nanjing Medical University, Nanjing 210029, China

【机构】 南京医科大学第一附属医院感染科南京医科大学基础医学院免疫学教研室南京医科大学公共卫生学院南京医科大学基础医学院病原生物学系南京医科大学第一附属医院感染科 江苏南京210029江苏南京210029

【摘要】 目的:分离人外周血CD4+CD25+Treg细胞,并检测其功能。方法:RT-PCR技术检测CD4+CD25+Treg细胞中Foxp3的mRNA表达;与CD8+T细胞和CD4+CD25-T细胞共同培养,或加入外源性IL-2及IL-4,检测其抑制功能;流式细胞术检测IFN-γ、IL-4和IL-10。结果:CD4+CD25+Treg细胞高表达Foxp3,主要分泌IL-10,能够抑制CD8+T细胞和CD4+CD25-T细胞的增殖,高浓度IL-2能够阻断CD4+CD25+Treg细胞的抑制功能。结论:CD4+CD25+Treg细胞是一群具有免疫抑制功能的调节性T细胞,这种抑制作用能够被高浓度IL-2阻断。

【Abstract】 AIM: To isolate CD4~+ CD25~+ Treg cells from human peripheral blood, and study their functional characteristics. METHODS: The expressions of Foxp3 in human CD4~+ CD25~+ Treg cells were test by RT-PCR. The regulatory properties of CD4~+ CD25~+ Treg cells were assessed by co-culturing with CD4~+ CD25~- T cells and CD8~+ T cells, or adding exogenous IL-2. The detection of intracellular cytokine production of IL-4, IL-10 and IFN-γ was done by flow cytometry. RESULTS: CD4~+ CD25~+ Treg cells highly expressed Foxp3 and mainly synthesized IL-10 that suppressed the proliferation of CD4~+ CD25~- T and CD8~+ T cells. Its suppressive function was reversed by high concentration of IL-2 and (or) IL-4. CONCLUSION: CD4~+ CD25~+ Treg cells exhibited a subpopulation of immunoregulatory T cells with suppressive function, which could be reversed by high concentration of IL-2.

【关键词】 CD4~+ CD25~+ Treg细胞免疫抑制IL-2
【Key words】 CD4~+ CD25~+ Treg cellimmune suppressionIL-2
【基金】 国家自然科学基金资助项目(No.30200232);南京医科大学创新基金资助项目(No.Cx200006)
  • 【文献出处】 细胞与分子免疫学杂志 ,Chinese Journal of Cellular and Molecular Immunology , 编辑部邮箱 ,2006年02期
  • 【分类号】R392.1
  • 【被引频次】32
  • 【下载频次】1571
节点文献中: 

本文链接的文献网络图示:

本文的引文网络