【摘要】 目的:研究混合二元醇对减轻戊二醛牛心包细胞毒性的作用。方法:牛心包分两组——A组:用0.625%戊二醛固定2w后用0.3%戊二醛保存;B组:牛心包用0.625%戊二醛固定2w后,用混合二元醇后处理并保存。C组:玻片对照组。二组心包均切成2cm×2cm,分别种植同样数目的(4×104)人胚肺成纤维细胞,培养7d后,分别计算每片心包上活细胞数。同时作光镜及扫描电镜观察。结果:①活细胞计数结果:A组、B组与对照组相比差异均有显著性(P<0.01)。B组与A组的活细胞数差异有显著性(P<0.01)。②光镜下:对照组细胞生长良好,A、B组均有死亡细胞,尤以A组为多。③扫描电镜:A组活细胞很少,且多为圆球形细胞;B组有较多的细胞覆盖生长,可见细胞分裂相。结论:混合二元醇改性处理后的戊二醛牛心包细胞毒性明显降低。更多还原

【Abstract】 Objective:To research that post-treated with polydiol affect the cytotoxicity of glutaraldehyde tanned bovine pericardium (G-tBP). Methods:Two groups of variouly preserved bovine pericardium tissue samples were used: Group A: Pericardium tanned with 0.625% glutaraldehyd(eGA)for two weeks,then preserved in 0.3% GA; Group B: treated as group A, then post-treated with polydiol for two months; Group C: Glass was used as a control. Each group was 6 specimens. The tissue was cut into same size (2 cm×2 cm). Same amount (4×104) of human embryo lung (HEL) fibroblast were seeding on the smooth surface of pericardium and glass sheet. After cultured for 7 days, living cells on each specimens were counted, and light microscopy and scanning electron microscopy (SEM) was examined. Result: The amount of living cells :Group A vs Group B, P<0.01; Group C vs Group A、B, P<0.01. Light microscopy showed the cells of group C were growing well. There were some dead cells in other groups, especially in group A. SEM showed there were only few cells on the surface of pericardium in group A. There were much more cells in group B. Conclusion: After glutaraldehyde tanned bovine pericardium post-treated with polydiol, its cytotoxicity reduces significantly.更多还原