【摘要】 对三孢布拉氏霉代谢产物的分析表明,该菌能够合成辅酶Q10,且正株合成辅酶Q10的能力高于负株。当正负菌株以5∶1混合培养时,辅酶Q10产量最高。三孢布拉氏霉合成辅酶Q10是遵循经典的辅酶Q10生物合成途径的,本实验选育到具有L-甲硫氨酸和D-酪氨酸或对氨基苯丙氨酸双重抗性标记的突变菌各一株,其辅酶Q10产量较出发菌株分别提高了76.7%及94.2%。更多还原

【Abstract】 The result from thin-layer chromatograph indicated that CoQ10 could be synthesis by Blakeslea trispora,and a higher concentration of CoQ10 was observed in the positive strain.A highest concentration of CoQ10 was detected in the mixture culture of positive strain and negative strain at the ratio of 5∶1.The results of metabolic pathway analysis shown that the CoQ10 synthesis pathway in Blakeslea trispora is followed the classic pathway of CoQ10 synthesis.Furthermore,two mutants that has dual resistant marker were isolated,and the yield of CoQ10 produce by these two strains increase 76.7% and 94.2%,respectively.更多还原