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产纤溶酶米曲霉的筛选及诱变育种

Screening and Mutagenesis of the Fibrinolytic Enzyme Producing Strain Asperillus oryzae

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【作者】 高占争赵允麟

【Author】 GAO Zhan-zheng,ZHAO Yun-lin(Key Laboratory of Industrial Biotechnology,Ministry of Education,Southern Yangtze University,Wuxi 214036,China)

【机构】 江南大学教育部工业微生物重点实验室江南大学教育部工业微生物重点实验室 江苏无锡214036江苏无锡214036

【摘要】 利用自己设计的筛选方法,从不同的酿造曲样中筛选出1株产纤溶酶酶活较高的米曲霉菌株SA-6,其产纤溶酶酶活为25 U/mL。SA-6经过紫外线6、0Co和亚硝基胍诱变,筛选得到突变株NA-25,其产酶酶活为63 U/mL,是出发菌株SA-6的2.52倍。突变株NA-25的发酵性状改变,其产纤溶酶的高峰比出发菌株迟了约10 h。

【Abstract】 A Asperillus Oryzae strain,SA-6 was isolated with different screening methods and the fibrinolytic enzyme activity was 25 U/mL.A mutant was then isolated after SA-6 was treated with UV,60Co and NTG and the relevant firbrinolytic enzyme activity was enhanced to 63 U/mL which was 1.52 times higher than that of SA-6.Comparing with the starting strain,the appearance time of the maximum fibrinolytic enzyme production of the mutant was delayed 10 hours.

【关键词】 纤溶酶米曲霉筛选诱变
【Key words】 fibrinolytic enzymeAsperillus Oryzaescreeningmutagenesis
  • 【文献出处】 食品与生物技术学报 ,Journal of Food Science and Biotechnology , 编辑部邮箱 ,2006年04期
  • 【分类号】TQ464.8
  • 【被引频次】12
  • 【下载频次】238
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