【摘要】 目的建立反相高效液相色谱法测定犬血浆罗哌卡因浓度的快速、简便的方法。方法色谱柱为Intertsil C18柱(4·6mm×250mm,5μm),流动相为甲醇-水相,体积比为50∶50,水相为0·005mol/L己烷基磺酸钠,冰醋酸调节pH至3·5,检测波长为215nm,流速为:1·4ml/min;柱温为室温;柱压为2800PSI,进样体积为10μl,灵敏度为0·02AUFS。结果罗哌卡因的检测浓度线性范围为0·1~25μg/ml(r=0·9992),最低检测浓度为0·05μg/ml,回归方程为^Y=0·1794X+0·3806,相关系数为r=0·9992,回收率为91·2%~93·6%,RSD为2·10%~3·40%。罗哌卡因测定的日内及日间精密度分别为1·35%~2·88%和1·80%~3·76%。结论本法操作简便、可靠、快捷、准确,适用于罗哌卡因类药物给药后血药浓度的检测。更多还原

【Abstract】 Objective To establish a Reversed-Phase high performance liquid chromatography method for determination of plasma ropivacaine in dog. Methods The concentration of ropivacaine was assayed on Intertsil C18 column （4.6mm×250mm,5μm）with a mobile phase consisting of methanol∶H₂O（50∶50）,the detection wavelength was 215nm and the flow rate was 1.4ml/min. the column temperature was 20℃ and the sensitivity was 0.02AUFS respectively. the volume of sample for detection is 10μl. Results the linear range was 0.1-25μg/ml（r=0.9982）.The recovery of ropivacaine was 91.2%-93.6%,RSD were 2.10%-3.40%（n=6）.The detection limit was 0.05μg/ml.the intra-day and interday precisions of assay for ropivacaine was 1.35%-2.88% and 1.80%-3.76%.Conclusion This method is simple,rapid,accurate and convenient for determination of the concentration of ropivacaine in dog plasma.更多还原